IF-GFP-ATRX | Addgene 45444 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

IF-GFP-ATRX

catalog :

45444

citations: 5

Reference
van Gerven M, Schild L, van Arkel J, Koopmans B, Broeils L, Meijs L, et al. Two opposing gene expression patterns within ATRX aberrant neuroblastoma. PLoS ONE. 2023;18:e0289084 pubmed publisher
Elbakry A, Juhasz S, Chan K, Lobrich M. ATRX and RECQ5 define distinct homologous recombination subpathways. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
Viswanath P, Batsios G, Mukherjee J, Gillespie A, Larson P, Luchman H, et al. Non-invasive assessment of telomere maintenance mechanisms in brain tumors. Nat Commun. 2021;12:92 pubmed publisher
Danussi C, Bose P, Parthasarathy P, Silberman P, Van Arnam J, Vitucci M, et al. Atrx inactivation drives disease-defining phenotypes in glioma cells of origin through global epigenomic remodeling. Nat Commun. 2018;9:1057 pubmed publisher
Valle García D, Griffiths L, Dyer M, Bernstein E, Recillas Targa F. The ATRX cDNA is prone to bacterial IS10 element insertions that alter its structure. Springerplus. 2014;3:222 pubmed publisher

product information

Catalog Number :

45444

Product Name :

IF-GFP-ATRX

article :

doi	10.1186/2193-1801-3-222
id	13599
pubmed_id	24834375

bacterial resistance :

Kanamycin

cloning :

backbone

pEGFP C2

backbone_mutation

backbone_origin

Clontech

backbone_size

4700

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Bacterial Expression

growth notes :

The EcoRI restriction pattern is different between the plasmid described in the paper and the plasmid available from Addgene. This difference does not effect the function of the plasmid.

growth strain :

ATRX expression vector

growth temp :

As ATRX is prone to insertion by IS10, you should sequence exon 8 and the entire sequence on occasion but especially after culturing in E. coli. A good primer for sequencing is GCCAACATGAACTCCAGTTATG.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	XbaI
cloning_site_5	BamHI
promoter	PCMV
sequencing_primer_3	EGFP C sequencing primer
sequencing_primer_5	EGFP N sequencing primer
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	JMS, MRX52, RAD54, RAD54L, XH2, XNP, ZNF-HX
gene	ATRX
id	546

genbank_ids

mutation

isoform 2, missing codon E124

name

ATRX

shRNA_sequence

size

7400

species

9606

Homo sapiens

10090

Mus musculus