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product summary
company name :
Addgene
product type :
cDNA
product name :
pLAMP1-mCherry
catalog :
45147
citations: 26
Reference
Wang Y, Wang C, Sylvers J, Segura T, Yuan F. Nanoenhancer for improving naked DNA electrotransfection In vivo. Front Bioeng Biotechnol. 2023;11:1181795 pubmed publisher
Li M, Tripathi Giesgen I, Schulman B, Baumeister W, Wilfling F. In situ snapshots along a mammalian selective autophagy pathway. Proc Natl Acad Sci U S A. 2023;120:e2221712120 pubmed publisher
Saito S, Ishikawa T, Ninagawa S, Okada T, Mori K. A motor neuron disease-associated mutation produces non-glycosylated Seipin that induces ER stress and apoptosis by inactivating SERCA2b. elife. 2022;11: pubmed publisher
Lee J, Xu Y, Saidi L, Xu M, ZINSMAIER K, Ye Y. Abnormal triaging of misfolded proteins by adult neuronal ceroid lipofuscinosis-associated DNAJC5/CSPα mutants causes lipofuscin accumulation. Autophagy. 2022;:1-20 pubmed publisher
da Silva R, Tay W, Ho F, Tanoto F, Chong K, Choo P, et al. Enterococcus faecalis alters endo-lysosomal trafficking to replicate and persist within mammalian cells. PLoS Pathog. 2022;18:e1010434 pubmed publisher
Liang J, Ruthel G, Freedman B, Harty R. WWOX-Mediated Degradation of AMOTp130 Negatively Affects Egress of Filovirus VP40 Virus-Like Particles. J Virol. 2022;96:e0202621 pubmed publisher
Xing Y, Wei X, Wang M, Liu Y, Sui Z, Wang X, et al. Stimulating TRPM7 suppresses cancer cell proliferation and metastasis by inhibiting autophagy. Cancer Lett. 2022;525:179-197 pubmed publisher
Radaszkiewicz T, Nosková M, Gömöryová K, Vondálová Blanárová O, Radaszkiewicz K, Pícková M, et al. RNF43 inhibits WNT5A-driven signaling and suppresses melanoma invasion and resistance to the targeted therapy. elife. 2021;10: pubmed publisher
Boutry M, Kim P. ORP1L mediated PI(4)P signaling at ER-lysosome-mitochondrion three-way contact contributes to mitochondrial division. Nat Commun. 2021;12:5354 pubmed publisher
Fu Y, Chen N, Wang Z, Luo S, Ding Y, Lu B. Degradation of lipid droplets by chimeric autophagy-tethering compounds. Cell Res. 2021;: pubmed publisher
Qi J, Xing Y, Liu Y, Wang M, Wei X, Sui Z, et al. MCOLN1/TRPML1 finely controls oncogenic autophagy in cancer by mediating zinc influx. Autophagy. 2021;:1-22 pubmed publisher
Santerre M, Arjona S, Allen C, Callen S, Buch S, Sawaya B. HIV-1 Vpr protein impairs lysosome clearance causing SNCA/alpha-synuclein accumulation in neurons. Autophagy. 2021;17:1768-1782 pubmed publisher
Wu H, Voeltz G. Reticulon-3 Promotes Endosome Maturation at ER Membrane Contact Sites. Dev Cell. 2021;56:52-66.e7 pubmed publisher
Mao M, Chang C, Pickar Oliver A, Cervia L, Wang L, Ji J, et al. Redirecting Vesicular Transport to Improve Nonviral Delivery of Molecular Cargo. Adv Biosyst. 2020;4:e2000059 pubmed publisher
Bayer C, Pitschelatow G, Hannemann N, Linde J, Reichard J, Pensold D, et al. DNA Methyltransferase 1 (DNMT1) Acts on Neurodegeneration by Modulating Proteostasis-Relevant Intracellular Processes. Int J Mol Sci. 2020;21: pubmed publisher
Wu X, Spence J, Das T, Yuan X, Chen C, Zhang Y, et al. Site-Specific Photo-Crosslinking Proteomics Reveal Regulation of IFITM3 Trafficking and Turnover by VCP/p97 ATPase. Cell Chem Biol. 2020;27:571-585.e6 pubmed publisher
Mutvei A, Nagiec M, Hamann J, Kim S, Vincent C, Blenis J. Rap1-GTPases control mTORC1 activity by coordinating lysosome organization with amino acid availability. Nat Commun. 2020;11:1416 pubmed publisher
Boutry M, Pierga A, Matusiak R, Branchu J, Houllegatte M, Ibrahim Y, et al. Loss of spatacsin impairs cholesterol trafficking and calcium homeostasis. Commun Biol. 2019;2:380 pubmed publisher
Szczurkowska J, Pischedda F, Pinto B, Managò F, Haas C, Summa M, et al. NEGR1 and FGFR2 cooperatively regulate cortical development and core behaviours related to autism disorders in mice. Brain. 2018;141:2772-2794 pubmed publisher
Oikonomidi I, Burbridge E, Cavadas M, Sullivan G, Collis B, Naegele H, et al. iTAP, a novel iRhom interactor, controls TNF secretion by policing the stability of iRhom/TACE. elife. 2018;7: pubmed publisher
Jayatilaka H, Giri A, Karl M, Aifuwa I, Trenton N, Phillip J, et al. EB1 and cytoplasmic dynein mediate protrusion dynamics for efficient 3-dimensional cell migration. FASEB J. 2018;32:1207-1221 pubmed publisher
Salasova A, Yokota C, Potesil D, Zdrahal Z, Bryja V, Arenas E. A proteomic analysis of LRRK2 binding partners reveals interactions with multiple signaling components of the WNT/PCP pathway. Mol Neurodegener. 2017;12:54 pubmed publisher
Zhitomirsky B, Assaraf Y. Lysosomal accumulation of anticancer drugs triggers lysosomal exocytosis. Oncotarget. 2017;8:45117-45132 pubmed publisher
Nguyen T, Padman B, Usher J, Oorschot V, Ramm G, Lazarou M. Atg8 family LC3/GABARAP proteins are crucial for autophagosome-lysosome fusion but not autophagosome formation during PINK1/Parkin mitophagy and starvation. J Cell Biol. 2016;215:857-874 pubmed
Evans A, Blackburn J, Yin C, Heit B. Quantitative Efferocytosis Assays. Methods Mol Biol. 2017;1519:25-41 pubmed
Van Engelenburg S, Palmer A. Imaging type-III secretion reveals dynamics and spatial segregation of Salmonella effectors. Nat Methods. 2010;7:325-30 pubmed publisher
product information
Catalog Number :
45147
Product Name :
pLAMP1-mCherry
article :
doi10.1038/nmeth.1437
id6637
pubmed_id20228815
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1
backbone_mutationmodified to contain mCherry and be compatible with Gateway cloning
backbone_originInvitrogen
backbone_size5500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3BGH Reverse
sequencing_primer_5CMV Fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCD107a, LAMPA, LGP120
geneLAMP1
id3916
genbank_ids
mutation
nameLAMP1-mCherry
shRNA_sequence
size2000
species
9606
Homo sapiens
100
Synthetic
tags
locationC terminal on insert
tagmCherry
resistance markers :
1227
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA