This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCI-neo:Beta2meGFP
catalog :
45096
citations: 1
Reference
Srinivasan R, Pantoja R, Moss F, Mackey E, Son C, Miwa J, et al. Nicotine up-regulates alpha4beta2 nicotinic receptors and ER exit sites via stoichiometry-dependent chaperoning. J Gen Physiol. 2011;137:59-79 pubmed publisher
product information
Catalog Number :
45096
Product Name :
pCI-neo:Beta2meGFP
article :
doi10.1085/jgp.201010532
id5157
pubmed_id21187334
bacterial resistance :
Ampicillin
cloning :
backbonepCI-neo
backbone_mutation
backbone_originPromega
backbone_size5472
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Pi ; Henry A. Lester 2 was cloned into pCI-neo at the restriction site EcoRI
origin :
37
pi :
alt_names
beta2-mGFP
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3T3
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAcrb-2, Acrb2, C030030P04Rik, [b]2-nAchR
geneChrnb2
id11444
genbank_ids
mutationThere are strain differences between C57BJ/6 and 129 in Chrnb2 at position 380. In C57BJ/6 it is a glutamate and in 129 it is a lysine. All of the forms of 2 currently in the lab are of the 129 variant except the 2 with GFP which was changed to a glutamate when GFP was inserted.
namenAChR beta2
shRNA_sequence
size2235
species
10090
Mus musculus
tags
plasmid copy :
J. Stizel (University of Colorado, Boulder, CO)
resistance markers :
288
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA