This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRSV-PKImut-v2
catalog :
45067
citations: 1
Reference
Taylor E, Pantazaka E, Shelley K, Taylor C. Prostaglandin E2 Inhibits Histamine-Evoked Ca2+ Release in Human Aortic Smooth Muscle Cells through Hyperactive cAMP Signaling Junctions and Protein Kinase A. Mol Pharmacol. 2017;92:533-545 pubmed publisher
product information
Catalog Number :
45067
Product Name :
pRSV-PKImut-v2
article :
doi
id6618
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepRSV-link
backbone_mutationBackbone derived from pRSV-globin
backbone_originMaurer lab
backbone_size4265
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Derived from RSV-PKI constructed by the Maurer lab and described in Day et al., J. Biol. Chem. 264, 431-436, 1989. Altered to add a Kozak consensus translation initiation sequence and also the ori was changed to yield high copy number in E coli.
growth strain :
Expression of intactive PKI (PKA inhibitor) mutant
origin :
37
pi :
alt_names
PKI alpha
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5HindIII
promoterRSV
sequencing_primer_3
sequencing_primer_5CGATACAATAAACGCCATTTGA
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
M23079.1
mutationchanged Arg 20 & 21 to Glycines
namecAMP-dependent protein kinase inhibitor alpha
shRNA_sequence
size250
species
100
Synthetic
tags
resistance markers :
1631
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA