This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
NL4-3 mCherry Luciferase
catalog :
44965
citations: 10
Reference
Charv xe1 tov xe1 S, Motais B, Czapla J, Cicho x144 T, Smolarczyk R, Walek Z, et al. Novel Local "Off-the-Shelf" Immunotherapy for the Treatment of Myeloma Bone Disease. Cells. 2023;12: pubmed publisher
Moon J, Jung Y, Moon S, Hwang J, Kim S, Kim M, et al. Production and characterization of lentivirus vector-based SARS-CoV-2 pseudoviruses with dual reporters: Evaluation of anti-SARS-CoV-2 viral effect of Korean Red Ginseng. J Ginseng Res. 2023;47:123-132 pubmed publisher
Shah M, Ung Moon S, Hyun Kim J, Thanh Thao T, Goo Woo H. SARS-CoV-2 pan-variant inhibitory peptides deter S1-ACE2 interaction and neutralize delta and omicron pseudoviruses. Comput Struct Biotechnol J. 2022;20:2042-2056 pubmed publisher
Peng L, Hu Y, Mankowski M, Ren P, Chen R, Wei J, et al. Monospecific and bispecific monoclonal SARS-CoV-2 neutralizing antibodies that maintain potency against B.1.617. Nat Commun. 2022;13:1638 pubmed publisher
Yang H, Cao J, Lin X, Yue J, Zieneldien T, Kim J, et al. Developing an Effective Peptide-Based Vaccine for COVID-19: Preliminary Studies in Mice Models. Viruses. 2022;14: pubmed publisher
Qiang M, Ma P, Li Y, Liu H, Harding A, Min C, et al. Neutralizing Antibodies to SARS-CoV-2 Selected from a Human Antibody Library Constructed Decades Ago. Adv Sci (Weinh). 2021;:e2102181 pubmed publisher
Yue J, Jin W, Yang H, Faulkner J, Song X, Qiu H, et al. Heparan Sulfate Facilitates Spike Protein-Mediated SARS-CoV-2 Host Cell Invasion and Contributes to Increased Infection of SARS-CoV-2 G614 Mutant and in Lung Cancer. Front Mol Biosci. 2021;8:649575 pubmed publisher
Motais B, Charvátová S, Walek Z, Hrdinka M, Smolarczyk R, Cichon T, et al. Selection, Expansion, and Unique Pretreatment of Allogeneic Human Natural Killer Cells with Anti-CD38 Monoclonal Antibody for Efficient Multiple Myeloma Treatment. Cells. 2021;10: pubmed publisher
Contreras X, Salifou K, Sanchez G, Helsmoortel M, Beyne E, Bluy L, et al. Nuclear RNA surveillance complexes silence HIV-1 transcription. PLoS Pathog. 2018;14:e1006950 pubmed publisher
Lassen K, Hebbeler A, Bhattacharyya D, Lobritz M, Greene W. A flexible model of HIV-1 latency permitting evaluation of many primary CD4 T-cell reservoirs. PLoS ONE. 2012;7:e30176 pubmed publisher
product information
Catalog Number :
44965
Product Name :
NL4-3 mCherry Luciferase
article :
doi10.1371/journal.pone.0030176
id6594
pubmed_id22291913
bacterial resistance :
Ampicillin
cloning :
backbonepNLENG1
backbone_mutation
backbone_originAIDS Research and Reference Reagent Program
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Lentiviral
growth notes :
Please note that the D212G mutation in mCherry has no effect on the function of this plasmid.
growth strain :
HIV dual reporter vector expressing mCh & Luc to simultaneously measure the # of cells containing reactivated latent provirus and the overall strength of viral transcriptional response in these cells.
origin :
30
pi :
alt_names
HIV 1 reporter virus
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5BamHI
promoter
sequencing_primer_3from pNLENG1 3'
sequencing_primer_5from pNLENG1 5'
site_3_destroyed1
site_5_destroyed
entrez_gene
genbank_ids
mutationS34C mutation in Nef
namemCherry, Luciferase, IRES
shRNA_sequence
size
species
32644
Other
Lentivirus, Human specific
tags
locationC terminal on insert
tagmCherry with D212G
locationC terminal on insert
tagLuciferase, IRES
plasmid copy :
pNL4-3 obtained from the AIDS Research and Reference Reagent Program pSicoRMS2 from Matt Spindler and Bruce Conklin, Gladstone Institute of Cardiovascular Disease
resistance markers :
176
tags :
Unknown
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA