This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCas9D10A_GFP
catalog :
44720
citations: 18
Reference
Kamat K, Inamdar M. Generation of OCIAD2 homozygous knockout (BJNhem20-OCIAD2-CRISPR-33) and heterozygous knockout (BJNhem20-OCIAD2-CRISPR-40) human embryonic stem cell lines using CRISPR-Cas9 mediated targeting. Stem Cell Res. 2023;67:103026 pubmed publisher
White J, Thompson D, Koch K, Kiriazov B, Beck A, van der Heide D, et al. AP-2alpha-mediated activation of E2F and EZH2 drives melanoma metastasis. Cancer Res. 2021;: pubmed publisher
Keen K, Petersen A, Figueroa A, Fordyce B, Shin J, Yadav R, et al. Physiological Characterization and Transcriptomic Properties of GnRH Neurons Derived From Human Stem Cells. Endocrinology. 2021;162: pubmed publisher
Jansen E, van Bakel N, Benedict B, Olde Loohuis N, Hafmans T, Chim S, et al. Novel vertebrate- and brain-specific driver of neuronal outgrowth. Prog Neurobiol. 2021;202:102069 pubmed publisher
Beck A, Cho E, White J, Paemka L, Li T, Gu V, et al. AP-2α Regulates S-Phase and Is a Marker for Sensitivity to PI3K Inhibitor Buparlisib in Colon Cancer. Mol Cancer Res. 2021;19:1156-1167 pubmed publisher
Jackson M, Fidanza A, Taylor A, Rybtsov S, Axton R, Kydonaki M, et al. Modulation of APLNR Signaling Is Required during the Development and Maintenance of the Hematopoietic System. Stem Cell Reports. 2021;16:727-740 pubmed publisher
Fontana D, Mauri M, Renso R, Docci M, Crespiatico I, Røst L, et al. ETNK1 mutations induce a mutator phenotype that can be reverted with phosphoethanolamine. Nat Commun. 2020;11:5938 pubmed publisher
Xiong M, Tao Y, Gao Q, Feng B, Yan W, Zhou Y, et al. Human Stem Cell-Derived Neurons Repair Circuits and Restore Neural Function. Cell Stem Cell. 2021;28:112-126.e6 pubmed publisher
Cardenas Diaz F, Osorio Quintero C, Diaz Miranda M, Kishore S, LEAVENS K, Jobaliya C, et al. Modeling Monogenic Diabetes using Human ESCs Reveals Developmental and Metabolic Deficiencies Caused by Mutations in HNF1A. Cell Stem Cell. 2019;25:273-289.e5 pubmed publisher
Zaslavsky K, Zhang W, McCready F, Rodrigues D, Deneault E, Loo C, et al. SHANK2 mutations associated with autism spectrum disorder cause hyperconnectivity of human neurons. Nat Neurosci. 2019;22:556-564 pubmed publisher
Woodbury Smith M, Deneault E, Yuen R, Walker S, Zarrei M, Pellecchia G, et al. Mutations in RAB39B in individuals with intellectual disability, autism spectrum disorder, and macrocephaly. Mol Autism. 2017;8:59 pubmed publisher
Lee J, Krivega I, Dale R, Dean A. The LDB1 Complex Co-opts CTCF for Erythroid Lineage-Specific Long-Range Enhancer Interactions. Cell Rep. 2017;19:2490-2502 pubmed publisher
Lai F, Lau S, Wong J, Gui H, Wang R, Zhou T, et al. Correction of Hirschsprung-Associated Mutations in Human Induced Pluripotent Stem Cells Via Clustered Regularly Interspaced Short Palindromic Repeats/Cas9, Restores Neural Crest Cell Function. Gastroenterology. 2017;153:139-153.e8 pubmed publisher
Bressan R, Dewari P, Kalantzaki M, Gangoso E, Matjusaitis M, Garcia Diaz C, et al. Efficient CRISPR/Cas9-assisted gene targeting enables rapid and precise genetic manipulation of mammalian neural stem cells. Development. 2017;144:635-648 pubmed publisher
Shetty D, Inamdar M. Generation of a heterozygous knockout human embryonic stem cell line for the OCIAD1 locus using CRISPR/CAS9 mediated targeting: BJNhem20-OCIAD1-CRISPR-39. Stem Cell Res. 2016;16:308-10 pubmed publisher
Courtney D, Moore J, Atkinson S, Maurizi E, Allen E, Pedrioli D, et al. CRISPR/Cas9 DNA cleavage at SNP-derived PAM enables both in vitro and in vivo KRT12 mutation-specific targeting. Gene Ther. 2016;23:108-12 pubmed publisher
Chen Y, Cao J, Xiong M, Petersen A, Dong Y, Tao Y, et al. Engineering Human Stem Cell Lines with Inducible Gene Knockout using CRISPR/Cas9. Cell Stem Cell. 2015;17:233-44 pubmed publisher
Ding Q, Regan S, Xia Y, Oostrom L, Cowan C, Musunuru K. Enhanced efficiency of human pluripotent stem cell genome editing through replacing TALENs with CRISPRs. Cell Stem Cell. 2013;12:393-4 pubmed publisher
product information
Catalog Number :
44720
Product Name :
pCas9D10A_GFP
article :
doi10.1016/j.stem.2013.03.006
id6547
pubmed_id23561441
bacterial resistance :
Ampicillin
cloning :
backbonepCAG
backbone_mutation
backbone_origin
backbone_size4200
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
CRISPR
growth notes :
For more information on Musunuru Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/Musunuru/
growth strain :
Co-expression of human codon-optimized Cas9 (D10A) mutant nickase and GFP, plasmid optimized for expression in human pluripotent stem cells
origin :
37
pi :
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterCAG
sequencing_primer_3M13R
sequencing_primer_5ggctctagtgcctctgctaacc
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutationD10A mutation- nickase mutant
nameCas9D10A-2A-GFP
shRNA_sequence
size4926
species
100
Synthetic
tags
locationC terminal on insert
tag2A-GFP
plasmid copy :
George Church
resistance markers :
1454
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA