This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLKO-shBRCA1 #1
catalog :
44594
citations: 4
Reference
Garc xed a Puga M, Saenz Anto xf1 anzas A, Gerenu G, Arrieta Legorburu A, Fern xe1 ndez Torr xf3 n R, Zulaica M, et al. Senescence plays a role in myotonic dystrophy type 1. JCI Insight. 2022;7: pubmed publisher
Li J, Shu X, Xu J, Su S, Chan U, Mo L, et al. S100A9-CXCL12 activation in BRCA1-mutant breast cancer promotes an immunosuppressive microenvironment associated with resistance to immunotherapy. Nat Commun. 2022;13:1481 pubmed publisher
Lei J, Lee M, Miao K, Huang Z, Yao Z, Zhang A, et al. Activation of FGFR2 Signaling Suppresses BRCA1 and Drives Triple-Negative Mammary Tumorigenesis That is Sensitive to Immunotherapy. Adv Sci (Weinh). 2021;8:e2100974 pubmed publisher
Okamoto K, Bartocci C, Ouzounov I, Diedrich J, Yates J, Denchi E. A two-step mechanism for TRF2-mediated chromosome-end protection. Nature. 2013;494:502-5 pubmed publisher
product information
Catalog Number :
44594
Product Name :
pLKO-shBRCA1 #1
article :
doi10.1038/nature11873
id6384
pubmed_id23389450
bacterial resistance :
Ampicillin
cloning :
backbonepLKO.1 puro
backbone_mutation
backbone_originRobert Weinberg (Addgene plasmid #8453)
backbone_size7032
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
RNAi
growth notes :
Observed knockdown = 85%
origin :
37
pi :
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterU6
sequencing_primer_3
sequencing_primer_5LKO.1 5'
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneBrca1
id12189
genbank_ids
mutation
nameBRCA1
shRNA_sequenceAATTCAGAGCCAGATTATGTA
size
species
10090
Mus musculus
tags
resistance markers :
1499
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA