This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
K18iresCreER-T2
catalog :
44580
citations: 3
Reference
Seo S, Son J, Lee J, Kim N, Yoo E, Kang A, et al. Development of transgenic models susceptible and resistant to SARS-CoV-2 infection in FVB background mice. PLoS ONE. 2022;17:e0272019 pubmed publisher
Shoshkes Carmel M, WANG Y, Wangensteen K, Tóth B, Kondo A, Massasa E, et al. Subepithelial telocytes are an important source of Wnts that supports intestinal crypts. Nature. 2018;557:242-246 pubmed publisher
Wen F, Cecena G, Munoz Ritchie V, Fuchs E, Chambon P, Oshima R. Expression of conditional cre recombinase in epithelial tissues of transgenic mice. Genesis. 2003;35:100-6 pubmed
product information
Catalog Number :
44580
Product Name :
K18iresCreER-T2
article :
doi10.1002/gene.10169
id6509
pubmed_id12533792
bacterial resistance :
Ampicillin
cloning :
backbonepGEM-1
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mouse Targeting
growth notes :
Cre recombinase-mutant estrogen receptor fusion (CreERT2) in K18 gene-ires vector for tamoxifen-dependent Cre activity confined to epithelial tissues. This is the full length K18 gene with an IRES inserted downstream of sixth exon TGA and before the polyA signal. Please note that the partial plasmid sequence available for this plasmid is missing the human ERT2 sequence. This has been indicated in the partial sequence by a string of 10 N's at bp#7941-7950 immediately after the Cre sequence. See Addgene's sequencing result with the Cre-F primer for more information about this region. Human K18 gene from pGC1853 (Addgene #44619).
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NA
cloning_site_5NA
promoter
sequencing_primer_3T7
sequencing_primer_5Sp6
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCK-18, CYK18, K18
geneKRT18
id3875
genbank_ids
mutation
namekeratin 18 gene
shRNA_sequence
size
species
9606
Homo sapiens
tags
location
tagIRES_CreER-T2
plasmid copy :
CreER was provided by Dr. Pierre Chambon.
resistance markers :
1519
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA