pDN-G1TGt | Addgene 44508 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pDN-G1TGt

catalog :

44508

citations: 1

Reference
Nevozhay D, Adams R, Murphy K, Josic K, BALAZSI G. Negative autoregulation linearizes the dose-response and suppresses the heterogeneity of gene expression. Proc Natl Acad Sci U S A. 2009;106:5123-8 pubmed publisher

product information

Catalog Number :

44508

Product Name :

pDN-G1TGt

article :

doi	10.1073/pnas.0809901106
id	6308
pubmed_id	19279212

bacterial resistance :

Ampicillin

cloning :

backbone

pRS404

backbone_mutation

CYC1 transcriptional terminator present downstream of inserts (between XhoI and PvuII sites). Additional, nonfunctional tetracycline repressor (tetR) fragment present between SacI and SpeI sites.

backbone_origin

Stratagene

backbone_size

4274

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Yeast Expression

Synthetic Biology

Other

Expression regulator/reporter

origin :

pi :

alt_names

Yeast GAL1 promoter with 2XtetO2

cloning

clone_method	Restriction Enzyme
cloning_site_3	BamHI
cloning_site_5	AgeI
promoter	PGal1-D12 promoter
sequencing_primer_3	Tet-R (GGCGAGTTTACGGGTTGTTA)
sequencing_primer_5	Gal1ProF2 (CGAAGCGATGATTTTTGATC)
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	YBR020W
gene	GAL1
id	852308

genbank_ids

mutation

Two tandem tet operators (2XtetO2) downstream of the GAL1 TATA box

name

PGal1-D12 promoter

shRNA_sequence

size

450

species

4932

Saccharomyces cerevisiae

100

Synthetic