This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pwtCas9-bacteria
catalog :
44250
citations: 17
Reference
Herring Nicholas A, Dimig H, Roesing M, Josephs E. Selection of extended CRISPR RNAs with enhanced targeting and specificity. Commun Biol. 2024;7:86 pubmed publisher
Zhu Y, Liu Z, Gui L, Yun W, Mao C, Deng R, et al. Inhibition of CXorf56 promotes PARP inhibitor-induced cytotoxicity in triple-negative breast cancer. NPJ Breast Cancer. 2023;9:34 pubmed publisher
Herring Nicholas A, Dimig H, Roesing M, Josephs E. Selection of Extended CRISPR RNAs with Enhanced Targeting and Specificity. bioRxiv. 2023;: pubmed publisher
Burian J, Libis V, Hernandez Y, Guerrero Porras L, Ternei M, Brady S. High-throughput retrieval of target sequences from complex clone libraries using CRISPRi. Nat Biotechnol. 2022;: pubmed publisher
Tsuji A, Takei Y, Azuma Y. Establishment of genetic tools for genomic DNA engineering of Halomonas sp. KM-1, a bacterium with potential for biochemical production. Microb Cell Fact. 2022;21:122 pubmed publisher
Liu X, Wang X, Sun B, Sun L. The Involvement of Thiamine Uptake in the Virulence of Edwardsiella piscicida. Pathogens. 2022;11: pubmed publisher
Contreras Llano L, Meyer C, Liu Y, Sarker M, Lim S, Longo M, et al. Holistic engineering of cell-free systems through proteome-reprogramming synthetic circuits. Nat Commun. 2020;11:3138 pubmed publisher
Audibert S, Tanguy Le Gac N, Rech J, Turlan C, Bouet J, Bystricky K, et al. Addressing the role of centromere sites in activation of ParB proteins for partition complex assembly. PLoS ONE. 2020;15:e0226472 pubmed publisher
Hamamoto K, Tokunaga T, Yagi N, Hirai I. Characterization of blaCTX-M-14 transposition from plasmid to chromosome in Escherichia coli experimental strain. Int J Med Microbiol. 2020;310:151395 pubmed publisher
Zheng K, Wang Y, Li N, Jiang F, Wu C, Liu F, et al. Highly efficient base editing in bacteria using a Cas9-cytidine deaminase fusion. Commun Biol. 2018;1:32 pubmed publisher
Dong H, Xiang H, Mu D, Wang D, Wang T. Exploiting a conjugative CRISPR/Cas9 system to eliminate plasmid harbouring the mcr-1 gene from Escherichia coli. Int J Antimicrob Agents. 2019;53:1-8 pubmed publisher
Liu F, Zheng K, Chen H, Liu Z. Capping-RACE: a simple, accurate, and sensitive 5' RACE method for use in prokaryotes. Nucleic Acids Res. 2018;46:e129 pubmed publisher
Mehrer C, Incha M, Politz M, Pfleger B. Anaerobic production of medium-chain fatty alcohols via a ?-reduction pathway. Metab Eng. 2018;48:63-71 pubmed publisher
Su T, Liu F, Gu P, Jin H, Chang Y, Wang Q, et al. A CRISPR-Cas9 Assisted Non-Homologous End-Joining Strategy for One-step Engineering of Bacterial Genome. Sci Rep. 2016;6:37895 pubmed publisher
Zhang K, Duan X, Wu J. Multigene disruption in undomesticated Bacillus subtilis ATCC 6051a using the CRISPR/Cas9 system. Sci Rep. 2016;6:27943 pubmed publisher
Briner A, Donohoue P, Gomaa A, Selle K, Slorach E, Nye C, et al. Guide RNA functional modules direct Cas9 activity and orthogonality. Mol Cell. 2014;56:333-9 pubmed publisher
Qi L, Larson M, Gilbert L, Doudna J, Weissman J, Arkin A, et al. Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression. Cell. 2013;152:1173-83 pubmed publisher
product information
Catalog Number :
44250
Product Name :
pwtCas9-bacteria
article :
doi10.1016/j.cell.2013.02.022
id6441
pubmed_id23452860
bacterial resistance :
Ampicillin
cloning :
backbonepUC19
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
CRISPR
growth notes :
For more information on Qi Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/qi/
growth strain :
aTc-inducible expression of wild-type Cas9 (S .pyogenes) for bacterial gene knockdown
origin :
37
pi :
Cas9shRNA_sequencesize4107species
S .pyogenes
tags
Enzymecloning_site_3<
/td>XhoIcloning_s
ite_5BglIIpr
omoterpLtetO-1d>sequencing_primer_3ggagatcct
tactcgagttaGTCACCTCCTAGCTGACTCAAATCAAT<
/td>sequencing_primer_5
d>agaaaagaattcaaaAGATCTaaagaggagaaa
GGATCTATGGATAAGAAATACTCAATAGG
site_3_destroyed<
/tr>site_5_destroyedtd>entre
z_genegenban
k_idsmutatio
nnamed>wild-type
alt_names
cloning
clone_methodRestriction
resistance markers :
1487
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA