This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pdCas9::BFP-humanized
catalog :
44247
citations: 6
Reference
Bruder M, Walji S, Aucoin M. Comparison of CRISPR-Cas9 Tools for Transcriptional Repression and Gene Disruption in the BEVS. Viruses. 2021;13: pubmed publisher
Xiao L, Merullo D, Koch T, Cao M, Co M, Kulkarni A, et al. Expression of FoxP2 in the basal ganglia regulates vocal motor sequences in the adult songbird. Nat Commun. 2021;12:2617 pubmed publisher
Qian Y, Chen C, Ma L, Wang Z, Wang L, Zuo L, et al. CD38 Deficiency Promotes Inflammatory Response through Activating Sirt1/NF-κB-Mediated Inhibition of TLR2 Expression in Macrophages. Mediators Inflamm. 2018;2018:8736949 pubmed publisher
McComb S, Aguadé Gorgorió J, Harder L, Marovca B, Cario G, Eckert C, et al. Activation of concurrent apoptosis and necroptosis by SMAC mimetics for the treatment of refractory and relapsed ALL. Sci Transl Med. 2016;8:339ra70 pubmed publisher
Chakraborty S, Ji H, Kabadi A, Gersbach C, Christoforou N, Leong K. A CRISPR/Cas9-based system for reprogramming cell lineage specification. Stem Cell Reports. 2014;3:940-7 pubmed publisher
Qi L, Larson M, Gilbert L, Doudna J, Weissman J, Arkin A, et al. Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression. Cell. 2013;152:1173-83 pubmed publisher
product information
Catalog Number :
44247
Product Name :
pdCas9::BFP-humanized
article :
doi10.1016/j.cell.2013.02.022
id6441
pubmed_id23452860
bacterial resistance :
Ampicillin
cloning :
backbonepMSCVpuro
backbone_mutation
backbone_originClontech
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
CRISPR
growth strain :
Expression of a catalytically inactive, human codon-optimized Cas9-BFP fusion under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown
origin :
37
pi :
alt_names
Catalytically inactive Cas9
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterMSCV LTR promoter
sequencing_primer_3TCCCTCATTAAGCTTGTGCCCCAG
sequencing_primer_5CTAGGCGCCGGAATTAGATCTCGCCACCATGGA
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutationD10A H840A (catalytically inactive)
namedCas9 fused to BFP
shRNA_sequence
size4881
species
9606
Homo sapiens
tags
locationC terminal on insert
tag2xNLS & tagBFP
resistance markers :
1487
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA