This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pdCas9-humanized
catalog :
44246
citations: 17
Reference
Zhang Y, Zhang X, Ba Z, Liang Z, Dring E, Hu H, et al. The fundamental role of chromatin loop extrusion in physiological V(D)J recombination. Nature. 2019;573:600-604 pubmed publisher
Gao D, Liang F. Chemical Inducible dCas9-Guided Editing of H3K27 Acetylation in Mammalian Cells. Methods Mol Biol. 2018;1767:429-445 pubmed publisher
Luanpitpong S, Chanthra N, Janan M, Poohadsuan J, Samart P, U Pratya Y, et al. Inhibition of O-GlcNAcase Sensitizes Apoptosis and Reverses Bortezomib Resistance in Mantle Cell Lymphoma through Modification of Truncated Bid. Mol Cancer Ther. 2018;17:484-496 pubmed publisher
Luo W, Galvan D, Woodard L, Dorset D, Levy S, Wilson M. Comparative analysis of chimeric ZFP-, TALE- and Cas9-piggyBac transposases for integration into a single locus in human cells. Nucleic Acids Res. 2017;45:8411-8422 pubmed publisher
Yamazaki T, Hatano Y, Handa T, Kato S, Hoida K, Yamamura R, et al. Targeted DNA methylation in pericentromeres with genome editing-based artificial DNA methyltransferase. PLoS ONE. 2017;12:e0177764 pubmed publisher
Mouillesseaux K, Wiley D, Saunders L, Wylie L, Kushner E, Chong D, et al. Notch regulates BMP responsiveness and lateral branching in vessel networks via SMAD6. Nat Commun. 2016;7:13247 pubmed publisher
Ma Y, Zhang J, Yin W, Zhang Z, Song Y, Chang X. Targeted AID-mediated mutagenesis (TAM) enables efficient genomic diversification in mammalian cells. Nat Methods. 2016;13:1029-1035 pubmed publisher
Liu X, Wu H, Ji X, Stelzer Y, Wu X, Czauderna S, et al. Editing DNA Methylation in the Mammalian Genome. Cell. 2016;167:233-247.e17 pubmed publisher
Liu Y, Chen Z, He A, Zhan Y, Li J, Liu L, et al. Targeting cellular mRNAs translation by CRISPR-Cas9. Sci Rep. 2016;6:29652 pubmed publisher
Jusiak B, Cleto S, Perez Piñera P, Lu T. Engineering Synthetic Gene Circuits in Living Cells with CRISPR Technology. Trends Biotechnol. 2016;34:535-547 pubmed publisher
Aouida M, Eid A, Ali Z, Cradick T, Lee C, Deshmukh H, et al. Efficient fdCas9 Synthetic Endonuclease with Improved Specificity for Precise Genome Engineering. PLoS ONE. 2015;10:e0133373 pubmed publisher
Piatek A, Ali Z, Baazim H, Li L, Abulfaraj A, Al Shareef S, et al. RNA-guided transcriptional regulation in planta via synthetic dCas9-based transcription factors. Plant Biotechnol J. 2015;13:578-89 pubmed publisher
Lawhorn I, Ferreira J, Wang C. Evaluation of sgRNA target sites for CRISPR-mediated repression of TP53. PLoS ONE. 2014;9:e113232 pubmed publisher
Gao X, Tsang J, Gaba F, Wu D, Lu L, Liu P. Comparison of TALE designer transcription factors and the CRISPR/dCas9 in regulation of gene expression by targeting enhancers. Nucleic Acids Res. 2014;42:e155 pubmed publisher
Waldrip Z, Byrum S, Storey A, Gao J, Byrd A, Mackintosh S, et al. A CRISPR-based approach for proteomic analysis of a single genomic locus. Epigenetics. 2014;9:1207-11 pubmed publisher
Zhao Y, Dai Z, Liang Y, Yin M, Ma K, He M, et al. Sequence-specific inhibition of microRNA via CRISPR/CRISPRi system. Sci Rep. 2014;4:3943 pubmed publisher
Qi L, Larson M, Gilbert L, Doudna J, Weissman J, Arkin A, et al. Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression. Cell. 2013;152:1173-83 pubmed publisher
product information
Catalog Number :
44246
Product Name :
pdCas9-humanized
article :
doi10.1016/j.cell.2013.02.022
id6441
pubmed_id23452860
bacterial resistance :
Ampicillin
cloning :
backbonepMSCVpuro
backbone_mutation
backbone_originClontech
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
CRISPR
Other
vector
growth notes :
The dCas9 contains an N612K mutation that does not effect function. For more information on Qi Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/qi/
growth strain :
Expression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown
origin :
37
pi :
alt_names
Catalytically inactive Cas9
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterMSCV LTR promoter
sequencing_primer_3CCGGTAGAATTCTATACCTTTCTCTTCT
sequencing_primer_5CCGGAATTAGATCTCGCCACCATGGAC
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutationD10A H840A (catalytically inactive)
namedead Cas9 with 3X NLS
shRNA_sequence
size4188
species
9606
Homo sapiens
tags
locationC terminal on insert
tag3x NLS
resistance markers :
1487
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA