This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
p6659 MSCV-IP N FlagHA HPV16E6
catalog :
44152
citations: 2
Reference
Periyasamy M, Singh A, Gemma C, Kranjec C, Farzan R, Leach D, et al. p53 controls expression of the DNA deaminase APOBEC3B to limit its potential mutagenic activity in cancer cells. Nucleic Acids Res. 2017;45:11056-11069 pubmed publisher
White E, Kramer R, Tan M, Hayes S, Harper J, Howley P. Comprehensive analysis of host cellular interactions with human papillomavirus E6 proteins identifies new E6 binding partners and reflects viral diversity. J Virol. 2012;86:13174-86 pubmed publisher
product information
Catalog Number :
44152
Product Name :
p6659 MSCV-IP N FlagHA HPV16E6
article :
doi10.1128/JVI.02172-12
id6279
pubmed_id23015706
bacterial resistance :
Ampicillin
cloning :
backboneMSCV-IP N-FlagHA
backbone_mutationRetroviral vector for Flag-HA-tagged HPV16 E6 expression. No starting ATG in viral ORF, translation begins from tag only. Puro resistance protein expressed from an IRES.
backbone_origin
backbone_size8100
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterMSCV LTR
sequencing_primer_3
sequencing_primer_55' CTACATCGTGACCTGGGAAGC 3'
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameHPV16E6
shRNA_sequence
size500
species
HPV16
tags
locationN terminal on backbone
tagFlag
locationN terminal on backbone
tagHA
plasmid copy :
Paul Lambert - University of Wisconsin, Madison
resistance markers :
209
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA