pcDNA3 mHIF-2 MYC (P405A/P530V/N851A) | Addgene 44027 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA3 mHIF-2 MYC (P405A/P530V/N851A)

catalog :

44027

citations: 5

Reference
Kunkemoeller B, Chen K, Lockhart S, Wang X, Rask Madsen C. The transcriptional coregulator CITED2 suppresses expression of IRS-2 and impairs insulin signaling in endothelial cells. Am J Physiol Endocrinol Metab. 2021;321:E252-E259 pubmed publisher
Browe D, Coleman C, Barry F, Elliman S. Hypoxia Activates the PTHrP -MEF2C Pathway to Attenuate Hypertrophy in Mesenchymal Stem Cell Derived Cartilage. Sci Rep. 2019;9:13274 pubmed publisher
Xie L, Yin A, Nichenko A, Beedle A, Call J, Yin H. Transient HIF2A inhibition promotes satellite cell proliferation and muscle regeneration. J Clin Invest. 2018;128:2339-2355 pubmed publisher
Gu Y, Cohn S, Christie A, McKenzie T, Wolff N, Do Q, et al. Modeling Renal Cell Carcinoma in Mice: Bap1 and Pbrm1 Inactivation Drive Tumor Grade. Cancer Discov. 2017;7:900-917 pubmed publisher
Hu C, Sataur A, Wang L, Chen H, Simon M. The N-terminal transactivation domain confers target gene specificity of hypoxia-inducible factors HIF-1alpha and HIF-2alpha. Mol Biol Cell. 2007;18:4528-42 pubmed

product information

Catalog Number :

44027

Product Name :

pcDNA3 mHIF-2 MYC (P405A/P530V/N851A)

article :

doi	10.1091/mbc.e06-05-0419
id	6413
pubmed_id	17804822

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5446

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

This plasmid encodes an oxygen-regulation insensitive (normoxia-stable and active) full-length murine HIF-2 . Full-length murine HIF-2 cDNA was generated by reverse transcription-PCR and inserted into a pcDNA3 expression vector by using primers incorporated with restriction sites. A 10-amino-acid c-myc tag was further inserted at the C terminus of the HIF-2 cDNAs by using a Pfu-PCR-based protocol. The same Pfu-PCR protocol was then used to change residues Proline 405, Proline 530 and Asparagine 851. The newly constructed plasmid was sequenced to confirm encoding of the correct protein. This plasmid has an A94V polymorphism that does not affect protein function. Alternate plasmid names: HIF-2 triple mutant (TM); pcDNA3 HIF-2 TM

origin :

pi :

alt_names

HIF-2

Hypoxia-inducible factor-2alpha

Epas1

cloning

clone_method	Restriction Enzyme
cloning_site_3	NotI
cloning_site_5	KpnI
promoter	CMV
sequencing_primer_3	BGH-rev; Sp6
sequencing_primer_5	CMV-F; T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	HIF-2alpha, HIF2A, HLF, HRF, MOP2, bHLHe73
gene	Epas1
id	13819

genbank_ids

AK158430.1

AAH57870.1

mutation

P405A, P530V, N851A

name

mHIF-2 (P405A/P530V/N851A)

shRNA_sequence

size

2600

species

10090

Mus musculus