This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
p3s-Cas9HC
catalog :
43945
citations: 30
Reference
Kwon J, Kim M, Hwang W, Jo A, Hwang G, Jung M, et al. Extru-seq: a method for predicting genome-wide Cas9 off-target sites with advantages of both cell-based and in vitro approaches. Genome Biol. 2023;24:4 pubmed publisher
Pardieck J, Harb M, Sakiyama Elbert S. A transgenic mouse embryonic stem cell line for puromycin selection of V0V interneurons from heterogenous induced cultures. Stem Cell Res Ther. 2022;13:131 pubmed publisher
Kwon T, Ra J, Lee S, Baek I, Khim K, Lee E, et al. Precision targeting tumor cells using cancer-specific InDel mutations with CRISPR-Cas9. Proc Natl Acad Sci U S A. 2022;119: pubmed publisher
Hathi D, Chanswangphuwana C, Cho N, Fontana F, Maji D, Ritchey J, et al. Ablation of VLA4 in multiple myeloma cells redirects tumor spread and prolongs survival. Sci Rep. 2022;12:30 pubmed publisher
Akter M, Cui H, Chen Y, Ding B. Generation of two induced pluripotent stem cell lines with heterozygous and homozygous GAG deletion in TOR1A gene from a healthy hiPSC line. Stem Cell Res. 2021;56:102536 pubmed publisher
Gartrell J, Mellado Largarde M, Clay M, Bahrami A, Sahr N, Sykes A, et al. SLFN11 is Widely Expressed in Pediatric Sarcoma and Induces Variable Sensitization to Replicative Stress Caused By DNA-Damaging Agents. Mol Cancer Ther. 2021;20:2151-2165 pubmed publisher
Kim E, Kim J, Choi B, Kweon J, Park S, Lee H, et al. AWP1 Restrains the Aggressive Behavior of Breast Cancer Cells Induced by TNF-α. Front Oncol. 2021;11:631469 pubmed publisher
Chen Y, Sasaki Y, DiAntonio A, Milbrandt J. SARM1 is required in human derived sensory neurons for injury-induced and neurotoxic axon degeneration. Exp Neurol. 2021;:113636 pubmed publisher
Shin H, See J, Kweon J, Kim H, Sung G, Park S, et al. Small-molecule inhibitors of histone deacetylase improve CRISPR-based adenine base editing. Nucleic Acids Res. 2021;: pubmed publisher
Freibaum B, Messing J, Yang P, Kim H, Taylor J. High-fidelity reconstitution of stress granules and nucleoli in mammalian cellular lysate. J Cell Biol. 2021;220: pubmed publisher
Shin H, Kweon J, Kim Y. Gene Manipulation Using Fusion Guide RNAs for Cas9 and Cas12a. Methods Mol Biol. 2021;2162:185-193 pubmed publisher
Kang S, Lee W, An J, Lee J, Kim Y, Kim H, et al. Prediction-based highly sensitive CRISPR off-target validation using target-specific DNA enrichment. Nat Commun. 2020;11:3596 pubmed publisher
Munroe M, Niero E, Fok W, Vessoni A, Jeong H, Brenner K, et al. Telomere dysfunction activates p53 and represses HNF4α expression leading to impaired human hepatocyte development and function. Hepatology. 2020;: pubmed publisher
Mondal B, Jin H, Kallappagoudar S, Sedkov Y, Martinez T, Sentmanat M, et al. The histone deacetylase complex MiDAC regulates a neurodevelopmental gene expression program to control neurite outgrowth. elife. 2020;9: pubmed publisher
Zeineldin M, Federico S, Chen X, Fan Y, Xu B, Stewart E, et al. MYCN amplification and ATRX mutations are incompatible in neuroblastoma. Nat Commun. 2020;11:913 pubmed publisher
Nickolls A, Lee M, Espinoza D, Szczot M, Lam R, Wang Q, et al. Transcriptional Programming of Human Mechanosensory Neuron Subtypes from Pluripotent Stem Cells. Cell Rep. 2020;30:932-946.e7 pubmed publisher
Nagati J, Xu M, Garcia T, Comerford S, Hammer R, Garcia J. A substitution mutation in a conserved domain of mammalian acetate-dependent acetyl CoA synthetase 2 results in destabilized protein and impaired HIF-2 signaling. PLoS ONE. 2019;14:e0225105 pubmed publisher
Wang B, Maxwell B, Joo J, Gwon Y, Messing J, Mishra A, et al. ULK1 and ULK2 Regulate Stress Granule Disassembly Through Phosphorylation and Activation of VCP/p97. Mol Cell. 2019;74:742-757.e8 pubmed publisher
Niwa Y, Kanda G, Yamada R, Shi S, Sunagawa G, Ukai Tadenuma M, et al. Muscarinic Acetylcholine Receptors Chrm1 and Chrm3 Are Essential for REM Sleep. Cell Rep. 2018;24:2231-2247.e7 pubmed publisher
Lee J, Jeong E, Lee J, Jung M, Shin E, Kim Y, et al. Directed evolution of CRISPR-Cas9 to increase its specificity. Nat Commun. 2018;9:3048 pubmed publisher
Suzuki T, Asami M, Patel S, Luk L, Tsai Y, Perry A. Switchable genome editing via genetic code expansion. Sci Rep. 2018;8:10051 pubmed publisher
Urick M, Bell D. In vitro effects of FBXW7 mutation in serous endometrial cancer: Increased levels of potentially druggable proteins and sensitivity to SI-2 and dinaciclib. Mol Carcinog. 2018;57:1445-1457 pubmed publisher
Cooper M, Choi J, Staser K, Ritchey J, Devenport J, Eckardt K, et al. An "off-the-shelf" fratricide-resistant CAR-T for the treatment of T cell hematologic malignancies. Leukemia. 2018;32:1970-1983 pubmed publisher
Sentmanat M, Peters S, Florian C, Connelly J, Pruett Miller S. A Survey of Validation Strategies for CRISPR-Cas9 Editing. Sci Rep. 2018;8:888 pubmed publisher
Kweon J, Jang A, Kim D, Yang J, Yoon M, Rim Shin H, et al. Fusion guide RNAs for orthogonal gene manipulation with Cas9 and Cpf1. Nat Commun. 2017;8:1723 pubmed publisher
Chen Y, Kim M, Chen X, Batista P, Aoyama S, Wilusz J, et al. Sensing Self and Foreign Circular RNAs by Intron Identity. Mol Cell. 2017;67:228-238.e5 pubmed publisher
Kim D, Lim K, Kim S, Yoon S, Kim K, Ryu S, et al. Genome-wide target specificities of CRISPR RNA-guided programmable deaminases. Nat Biotechnol. 2017;35:475-480 pubmed publisher
Xu H, Iyer N, Huettner J, Sakiyama Elbert S. A puromycin selectable cell line for the enrichment of mouse embryonic stem cell-derived V3 interneurons. Stem Cell Res Ther. 2015;6:220 pubmed publisher
Sung Y, Jin Y, Kim S, Lee H. Generation of knockout mice using engineered nucleases. Methods. 2014;69:85-93 pubmed publisher
Cho S, Kim S, Kim J, Kim J. Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease. Nat Biotechnol. 2013;31:230-2 pubmed publisher
product information
Catalog Number :
43945
Product Name :
p3s-Cas9HC
article :
doi10.1038/nbt.2507
id6389
pubmed_id23360966
bacterial resistance :
Ampicillin
cloning :
backbonepCDNA3.1
backbone_mutationPvuII digestion and re-ligation to delete non-essential element in p3-Cas9HC.
backbone_origininvitrogen
backbone_size3200
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
CRISPR
growth notes :
For more information on Kim Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/Kim/
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5HindIII
promoterCMV
sequencing_primer_3SP6
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameCas9
shRNA_sequence
size4170
species
S. pyogenes
tags
locationC terminal on insert
tagNLS
locationC terminal on insert
tagHA epitope
resistance markers :
1064
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA