MAP2-CD4 | Addgene 43916 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

MAP2-CD4

catalog :

43916

citations: 1

Reference
Addis R, Hsu F, Wright R, Dichter M, Coulter D, Gearhart J. Efficient conversion of astrocytes to functional midbrain dopaminergic neurons using a single polycistronic vector. PLoS ONE. 2011;6:e28719 pubmed publisher

product information

Catalog Number :

43916

Product Name :

MAP2-CD4

article :

doi	10.1371/journal.pone.0028719
id	6382
pubmed_id	22174877

bacterial resistance :

Ampicillin

cloning :

backbone

FU-MAP2-Gateway

backbone_mutation

backbone_origin

Addgene plasmid 43915

backbone_size

9600

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

The MAP2-GFP lentiviral reporter plasmid pGZ-hMAP2 was purchased from System Biosciences (SR10047PA-1). The MAP2 promoter sequence from this plasmid was then cloned along with the Gateway cassette of pEF-DEST51 into the backbone of FUdeltaGW-rtTA (Addgene plasmid 19780) following removal of the ubiquitin promoter-rtTA sequence to produce FU-MAP2-Gateway (Addgene plasmid #43915). This destination vector was subsequently used to generate MAP2-CD4 (human CD4 sequence cloned from pMACS4-IRESII, Miltenyi Biotec, Cambridge, MA). The V5 and His tags present in the vector backbone are not expressed with the CD4 insert.

growth temp :

Use recA cells, such as STBL3 (Invitrogen)

origin :

pi :

alt_names

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter	Human MAP2 promoter
sequencing_primer_3	WPRE-R
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CD4mut, IMD79, Leu-3, OKT4D, T4
gene	CD4
id	920

genbank_ids

NM_000616.4

NP_000607.1

mutation

name

CD4

shRNA_sequence

size

1300

species

9606

Homo sapiens