This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
p415-GalL-Cas9-CYC1t
catalog :
43804
citations: 13
Reference
Mendoza B, Zheng X, Clements J, Cotter C, Trinh C. Potency of CRISPR-Cas Antifungals Is Enhanced by Cotargeting DNA Repair and Growth Regulatory Machinery at the Genetic Level. ACS Infect Dis. 2023;9:2494-2503 pubmed publisher
Liu M, Lin Y, Guo J, DU M, Tao X, Gao B, et al. High-Level Production of Sesquiterpene Patchoulol in Saccharomyces cerevisiae. ACS Synth Biol. 2021;10:158-172 pubmed publisher
Barber J, Sezmis A, Woods L, Anderson T, Voss J, McDonald M. The evolution of coexistence from competition in experimental co-cultures of Escherichia coli and Saccharomyces cerevisiae. ISME J. 2020;: pubmed publisher
Mosbach V, Viterbo D, Descorps Declère S, Poggi L, Vaysse Zinkhöfer W, Richard G. Resection and repair of a Cas9 double-strand break at CTG trinucleotide repeats induces local and extensive chromosomal deletions. PLoS Genet. 2020;16:e1008924 pubmed publisher
Standage Beier K, Brookhouser N, Balachandran P, Zhang Q, Brafman D, Wang X. RNA-Guided Recombinase-Cas9 Fusion Targets Genomic DNA Deletion and Integration. CRISPR J. 2019;2:209-222 pubmed publisher
Shao Y, Lu N, Xue X, Qin Z. Creating functional chromosome fusions in yeast with CRISPR-Cas9. Nat Protoc. 2019;14:2521-2545 pubmed publisher
Laughery M, Mayes H, Pedroza I, Wyrick J. R-loop formation by dCas9 is mutagenic in Saccharomyces cerevisiae. Nucleic Acids Res. 2019;47:2389-2401 pubmed publisher
Dank A, Smid E, Notebaart R. CRISPR-Cas genome engineering of esterase activity in Saccharomyces cerevisiae steers aroma formation. BMC Res Notes. 2018;11:682 pubmed publisher
Després P, Dubé A, Nielly Thibault L, Yachie N, Landry C. Double Selection Enhances the Efficiency of Target-AID and Cas9-Based Genome Editing in Yeast. G3 (Bethesda). 2018;8:3163-3171 pubmed publisher
Casini A, Olivieri M, Petris G, Montagna C, Reginato G, Maule G, et al. A highly specific SpCas9 variant is identified by in vivo screening in yeast. Nat Biotechnol. 2018;36:265-271 pubmed publisher
Giersch R, Finnigan G. Method for Multiplexing CRISPR/Cas9 in Saccharomyces cerevisiae Using Artificial Target DNA Sequences. Bio Protoc. 2017;7: pubmed publisher
Fu B, St Onge R, Fire A, Smith J. Distinct patterns of Cas9 mismatch tolerance in vitro and in vivo. Nucleic Acids Res. 2016;44:5365-77 pubmed publisher
Dicarlo J, Norville J, Mali P, Rios X, Aach J, Church G. Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems. Nucleic Acids Res. 2013;41:4336-43 pubmed publisher
product information
Catalog Number :
43804
Product Name :
p415-GalL-Cas9-CYC1t
article :
doi10.1093/nar/gkt135
id6442
pubmed_id23460208
bacterial resistance :
Ampicillin
cloning :
backbonep415
backbone_mutation
backbone_origin
backbone_size6670
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
CRISPR
growth notes :
For more information on Church Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/church/
origin :
37
pi :
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterGalL
sequencing_primer_3T7 promoter
sequencing_primer_5T3 promoter
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameHuman Optimized S. pyogenes Cas9
shRNA_sequence
size4140
species
9606
Homo sapiens
4932
Saccharomyces cerevisiae
S.pyogenes
tags
plasmid copy :
Plasmid 41815 from Prashant Mali
resistance markers :
765
tags :
High Copy
terms :
LEU2
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA