This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCas9
catalog :
42876
citations: 69
Reference
Tao S, Hu C, Fang Y, Zhang H, Xu Y, Zheng L, et al. Targeted elimination of Vancomycin resistance gene vanA by CRISPR-Cas9 system. BMC Microbiol. 2023;23:380 pubmed publisher
Tao S, Chen H, Li N, Fang Y, Zhang H, Xu Y, et al. Elimination of blaKPC-2-mediated carbapenem resistance in Escherichia coli by CRISPR-Cas9 system. BMC Microbiol. 2023;23:310 pubmed publisher
Carmody C, Nugen S. Monomeric streptavidin phage display allows efficient immobilization of bacteriophages on magnetic particles for the capture, separation, and detection of bacteria. Sci Rep. 2023;13:16207 pubmed publisher
Meile S, Du J, Staubli S, Grossmann S, Koliwer Brandl H, Piffaretti P, et al. Engineered reporter phages for detection of Escherichia coli, Enterococcus, and Klebsiella in urine. Nat Commun. 2023;14:4336 pubmed publisher
Thakur P, Deb R, Pegu S, Parihar R, Niharika J, Jyoti Das P, et al. Characterization of piggery farm waste-borne bacterial transposable elements associated with antimicrobial resistance phenotypes. Comp Immunol Microbiol Infect Dis. 2023;98:102005 pubmed publisher
Jo Y, Park S, Gong G, Roh S, Yoo J, Ahn J, et al. Enhanced production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with modulated 3-hydroxyvalerate fraction by overexpressing acetolactate synthase in Cupriavidus necator H16. Int J Biol Macromol. 2023;242:125166 pubmed publisher
Li X, Bao N, Yan Z, Yuan X, Wang S, Xia P. Degradation of Antibiotic Resistance Genes by VADER with CRISPR-Cas Immunity. Appl Environ Microbiol. 2023;89:e0005323 pubmed publisher
Wang N, Zhang Y, Jiang F, Zhu D, Di C, Hu S, et al. Enhancer variants on chromosome 2p14 regulating SPRED2 and ACTR2 act as a signal amplifier to protect against rheumatoid arthritis. Am J Hum Genet. 2023;110:625-637 pubmed publisher
Nyerges A, Vinke S, Flynn R, Owen S, Rand E, Budnik B, et al. A swapped genetic code prevents viral infections and gene transfer. Nature. 2023;615:720-727 pubmed publisher
Jenson J, Li T, Du F, Ea C, Chen Z. Ubiquitin-like conjugation by bacterial cGAS enhances anti-phage defence. Nature. 2023;616:326-331 pubmed publisher
Huan Y, Torraca V, Brown R, Fa Arun J, Miles S, Oyarz xfa n D, et al. P1 Bacteriophage-Enabled Delivery of CRISPR-Cas9 Antimicrobial Activity Against Shigella flexneri. ACS Synth Biol. 2023;12:709-721 pubmed publisher
Collias D, Vialetto E, Yu J, Co K, Alm xe1 si x, R xfc ttiger A, et al. Systematically attenuating DNA targeting enables CRISPR-driven editing in bacteria. Nat Commun. 2023;14:680 pubmed publisher
Gong Z, Guo J, Li Q, Xie H. Molecular mechanism of lipopolysaccharide (LPS) in promoting biomineralization on bacterial surface. Biochim Biophys Acta Gen Subj. 2023;1867:130305 pubmed publisher
Wu C, Hu B, Wang L, Wu X, Gu H, Dong H, et al. Assessment of stromal SCD-induced drug resistance of PDAC using 3D-printed zPDX model chips. iScience. 2023;26:105723 pubmed publisher
Deng P, Jia J, Foxfire A, Baird S, Smith L, Lu S. A Polyketide Synthetase Gene Cluster Is Responsible for Antibacterial Activity of Burkholderia contaminans MS14. Phytopathology. 2023;113:11-20 pubmed publisher
Tahir H, Basit A, Tariq H, Haider Z, Ullah A, Hayat Z, et al. Coupling CRISPR/Cas9 and Lambda Red Recombineering System for Genome Editing of Salmonella Gallinarum and the Effect of ssaU Knock-Out Mutant on the Virulence of Bacteria. Biomedicines. 2022;10: pubmed publisher
Cao L, Meng Z, Tan J, Ying M, Bi M, Liu Y, et al. Self-assembled endogenous DNA nanoparticles for auto-release and expression of the eGFP gene in Bacillus subtilis. Commun Biol. 2022;5:1373 pubmed publisher
Nethery M, Hidalgo Cantabrana C, Roberts A, Barrangou R. CRISPR-based engineering of phages for in situ bacterial base editing. Proc Natl Acad Sci U S A. 2022;119:e2206744119 pubmed publisher
Huan Y, Fa Arun J, Wang B. The Role of O-antigen in P1 Transduction of Shigella flexneri and Escherichia coli with its Alternative S' Tail Fibre. J Mol Biol. 2022;434:167829 pubmed publisher
Walflor H, Lucena A, Tuon F, Medeiros L, Faoro H. Resensitization of Fosfomycin-Resistant Escherichia coli Using the CRISPR System. Int J Mol Sci. 2022;23: pubmed publisher
Srikant S, Guegler C, Laub M. The evolution of a counter-defense mechanism in a virus constrains its host range. elife. 2022;11: pubmed publisher
Zhang X, Zhang C, Liang C, Li B, Meng F, Ai Y. CRISPR-Cas9 Based Bacteriophage Genome Editing. Microbiol Spectr. 2022;10:e0082022 pubmed publisher
Basit A, Tahir H, Haider Z, Tariq H, Ullah A, Rehman S. CRISPR/Cas9-Based Deletion of SpvB Gene From Salmonella gallinarum Leads to Loss of Virulence in Chicken. Front Bioeng Biotechnol. 2022;10:885227 pubmed publisher
Hobbs S, Wein T, Lu A, Morehouse B, Schnabel J, Leavitt A, et al. Phage anti-CBASS and anti-Pycsar nucleases subvert bacterial immunity. Nature. 2022;605:522-526 pubmed publisher
Zhu D, Chen X, Zhou X, Hu S, Tuo X, Hao R, et al. An Osteoporosis Susceptibility Allele at 11p15 Regulates SOX6 Expression by Modulating TCF4 Chromatin Binding. J Bone Miner Res. 2022;37:1147-1155 pubmed publisher
Deb R, Chaudhary P, De S. CRISPR/cas9 cassette targeting Escherichia coli blaCTX-M specific gene of mastitis cow milk origin can alter the antibiotic resistant phenotype for cefotaxime. Anim Biotechnol. 2022;:1-6 pubmed publisher
Shukla R, Zvara x, Avramucz x, Biketova A, Nyerges A, Pusk xe1 s L, et al. inPOSE: A Flexible Toolbox for Chromosomal Cloning and Amplification of Bacterial Transgenes. Microorganisms. 2022;10: pubmed publisher
Askoura M, Almalki A, Lila A, Almansour K, Alshammari F, Khafagy E, et al. Alteration of Salmonella enterica Virulence and Host Pathogenesis through Targeting sdiA by Using the CRISPR-Cas9 System. Microorganisms. 2021;9: pubmed publisher
Karpov D, Goncharenko A, Usachev E, Vasina D, Divisenko E, Chalenko Y, et al. A Strategy for the Rapid Development of a Safe Vibrio cholerae Candidate Vaccine Strain. Int J Mol Sci. 2021;22: pubmed publisher
Behrmann M, Perera H, Hoang J, Venkat T, Visser B, Bates D, et al. Targeted chromosomal Escherichia coli:dnaB exterior surface residues regulate DNA helicase behavior to maintain genomic stability and organismal fitness. PLoS Genet. 2021;17:e1009886 pubmed publisher
Wang Y, Cao L, Bi M, Wang S, Chen M, Chen X, et al. Wobble Editing of Cre-box by Unspecific CRISPR/Cas9 Causes CCR Release and Phenotypic Changes in Bacillus pumilus. Front Chem. 2021;9:717609 pubmed publisher
Avramucz Á, Møller Olsen C, Grigonyte A, Paramalingam Y, Millard A, Sagona A, et al. Analysing Parallel Strategies to Alter the Host Specificity of Bacteriophage T7. Biology (Basel). 2021;10: pubmed publisher
D Souza R, Nguyen L, Pinto N, Lee H, Vu T, Kim H, et al. Role of AmpG in the resistance to β-lactam agents, including cephalosporins and carbapenems: candidate for a novel antimicrobial target. Ann Clin Microbiol Antimicrob. 2021;20:45 pubmed publisher
Dong S, Zhu D, Zhou X, Rong Y, Zeng M, Chen J, et al. An Intronic Risk SNP rs12454712 for Central Obesity Acts As an Allele-Specific Enhancer To Regulate BCL2 Expression. Diabetes. 2021;: pubmed publisher
Ellis N, Kim B, Tung J, Machner M. A multiplex CRISPR interference tool for virulence gene interrogation in Legionella pneumophila. Commun Biol. 2021;4:157 pubmed publisher
Xie Q, Li S, Zhao D, Ye L, Li Q, Zhang X, et al. Manipulating the position of DNA expression cassettes using location tags fused to dCas9 (Cas9-Lag) to improve metabolic pathway efficiency. Microb Cell Fact. 2020;19:229 pubmed publisher
Duong M, Carmody C, Ma Q, Peters J, Nugen S. Optimization of T4 phage engineering via CRISPR/Cas9. Sci Rep. 2020;10:18229 pubmed publisher
Wei C, Chen T, Zhang Y, Wang Y, Shi D, Jiang Z, et al. A Novel White-to-Blue Colony Formation Assay to Select for Optimized sgRNAs. Mol Biotechnol. 2021;63:1-12 pubmed publisher
Duong M, Carmody C, Nugen S. Phage-based biosensors: in vivo analysis of native T4 phage promoters to enhance reporter enzyme expression. Analyst. 2020;145:6291-6297 pubmed publisher
Ruotsalainen P, Given C, Penttinen R, Jalasvuori M. Beta-Lactam Sensitive Bacteria Can Acquire ESBL-Resistance via Conjugation after Long-Term Exposure to Lethal Antibiotic Concentration. Antibiotics (Basel). 2020;9: pubmed publisher
Liu H, Li H, Liang Y, Du X, Yang C, Yang L, et al. Phage-delivered sensitisation with subsequent antibiotic treatment reveals sustained effect against antimicrobial resistant bacteria. Theranostics. 2020;10:6310-6321 pubmed publisher
Wan P, Cui S, Ma Z, Chen L, Li X, Zhao R, et al. Reversal of mcr-1-Mediated Colistin Resistance in Escherichia coli by CRISPR-Cas9 System. Infect Drug Resist. 2020;13:1171-1178 pubmed publisher
Soonsanga S, Luxananil P, Promdonkoy B. Modulation of Cas9 level for efficient CRISPR-Cas9-mediated chromosomal and plasmid gene deletion in Bacillus thuringiensis. Biotechnol Lett. 2020;42:625-632 pubmed publisher
Ziegler H, Nellen W. CRISPR-Cas experiments for schools and the public. Methods. 2020;172:86-94 pubmed publisher
Szili P, Draskovits G, Revesz T, Bogár F, Balogh D, Martinek T, et al. Rapid evolution of reduced susceptibility against a balanced dual-targeting antibiotic through stepping-stone mutations. Antimicrob Agents Chemother. 2019;: pubmed publisher
Møller Olsen C, Ho S, Shukla R, Fehér T, Sagona A. Engineered K1F bacteriophages kill intracellular Escherichia coli K1 in human epithelial cells. Sci Rep. 2018;8:17559 pubmed publisher
Li Y, Yan F, Wu H, Li G, Han Y, Ma Q, et al. Multiple-step chromosomal integration of divided segments from a large DNA fragment via CRISPR/Cas9 in Escherichia coli. J Ind Microbiol Biotechnol. 2019;46:81-90 pubmed publisher
Leenay R, Vento J, Shah M, Martino M, Leulier F, Beisel C. Genome Editing with CRISPR-Cas9 in Lactobacillus plantarum Revealed That Editing Outcomes Can Vary Across Strains and Between Methods. Biotechnol J. 2019;14:e1700583 pubmed publisher
Martino M, Joncour P, Leenay R, Gervais H, Shah M, Hughes S, et al. Bacterial Adaptation to the Host's Diet Is a Key Evolutionary Force Shaping Drosophila-Lactobacillus Symbiosis. Cell Host Microbe. 2018;24:109-119.e6 pubmed publisher
Xiong B, Li Z, Liu L, Zhao D, Zhang X, Bi C. Genome editing of Ralstonia eutropha using an electroporation-based CRISPR-Cas9 technique. Biotechnol Biofuels. 2018;11:172 pubmed publisher
Payne P, Geyrhofer L, Barton N, Bollback J. CRISPR-based herd immunity can limit phage epidemics in bacterial populations. elife. 2018;7: pubmed publisher
Sato o Y, Hisatsune J, Yu L, Sakuma T, Yamamoto T, Sugai M. Tailor-made gene silencing of Staphylococcus aureus clinical isolates by CRISPR interference. PLoS ONE. 2018;13:e0185987 pubmed publisher
Marshall R, Maxwell C, Collins S, Jacobsen T, Luo M, Begemann M, et al. Rapid and Scalable Characterization of CRISPR Technologies Using an E. coli Cell-Free Transcription-Translation System. Mol Cell. 2018;69:146-157.e3 pubmed publisher
Oesterle S, Gerngross D, Schmitt S, Roberts T, Panke S. Efficient engineering of chromosomal ribosome binding site libraries in mismatch repair proficient Escherichia coli. Sci Rep. 2017;7:12327 pubmed publisher
Wu M, Sung L, Li H, Huang C, Hu Y. Combining CRISPR and CRISPRi Systems for Metabolic Engineering of E. coli and 1,4-BDO Biosynthesis. ACS Synth Biol. 2017;6:2350-2361 pubmed publisher
Zhu X, Zhao D, Qiu H, Fan F, Man S, Bi C, et al. The CRISPR/Cas9-facilitated multiplex pathway optimization (CFPO) technique and its application to improve the Escherichia coli xylose utilization pathway. Metab Eng. 2017;43:37-45 pubmed publisher
Umenhoffer K, Draskovits G, Nyerges Ã, Karcagi I, Bogos B, Tímár E, et al. Genome-Wide Abolishment of Mobile Genetic Elements Using Genome Shuffling and CRISPR/Cas-Assisted MAGE Allows the Efficient Stabilization of a Bacterial Chassis. ACS Synth Biol. 2017;6:1471-1483 pubmed publisher
Lemay M, Tremblay D, Moineau S. Genome Engineering of Virulent Lactococcal Phages Using CRISPR-Cas9. ACS Synth Biol. 2017;6:1351-1358 pubmed publisher
Srinivas S, Cronan J. An Eight-Residue Deletion in Escherichia coli FabG Causes Temperature-Sensitive Growth and Lipid Synthesis Plus Resistance to the Calmodulin Inhibitor Trifluoperazine. J Bacteriol. 2017;199: pubmed publisher
Zhao D, Yuan S, Xiong B, Sun H, Ye L, Li J, et al. Development of a fast and easy method for Escherichia coli genome editing with CRISPR/Cas9. Microb Cell Fact. 2016;15:205 pubmed
Su T, Liu F, Gu P, Jin H, Chang Y, Wang Q, et al. A CRISPR-Cas9 Assisted Non-Homologous End-Joining Strategy for One-step Engineering of Bacterial Genome. Sci Rep. 2016;6:37895 pubmed publisher
Goshi N, Narenji A, Bui C, Mokili J, Kassegne S. Investigation Into the Effects of Nucleotide Content on the Electrical Characteristics of DNA Plasmid Molecular Wires. IEEE Trans Nanobioscience. 2016;15:585-594 pubmed publisher
Li H, Shen C, Huang C, Sung L, Wu M, Hu Y. CRISPR-Cas9 for the genome engineering of cyanobacteria and succinate production. Metab Eng. 2016;38:293-302 pubmed publisher
Chung M, Yeh I, Sung L, Wu M, Chao Y, Ng I, et al. Enhanced integration of large DNA into E. coli chromosome by CRISPR/Cas9. Biotechnol Bioeng. 2017;114:172-183 pubmed publisher
Westbrook A, Moo Young M, Chou C. Development of a CRISPR-Cas9 Tool Kit for Comprehensive Engineering of Bacillus subtilis. Appl Environ Microbiol. 2016;82:4876-95 pubmed publisher
Kim J, Cho D, Park M, Chung W, Shin D, Ko K, et al. CRISPR/Cas9-Mediated Re-Sensitization of Antibiotic-Resistant Escherichia coli Harboring Extended-Spectrum β-Lactamases. J Microbiol Biotechnol. 2016;26:394-401 pubmed publisher
Bryson A, Hwang Y, Sherrill Mix S, Wu G, Lewis J, Black L, et al. Covalent Modification of Bacteriophage T4 DNA Inhibits CRISPR-Cas9. MBio. 2015;6:e00648 pubmed publisher
Pyne M, Moo Young M, Chung D, Chou C. Coupling the CRISPR/Cas9 System with Lambda Red Recombineering Enables Simplified Chromosomal Gene Replacement in Escherichia coli. Appl Environ Microbiol. 2015;81:5103-14 pubmed publisher
Jiang W, Bikard D, Cox D, Zhang F, Marraffini L. RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Nat Biotechnol. 2013;31:233-9 pubmed publisher
product information
Catalog Number :
42876
Product Name :
pCas9
article :
doi10.1038/nbt.2508
id6312
pubmed_id23360965
bacterial resistance :
Chloramphenicol
cloning :
backbonepACYC184
backbone_mutation
backbone_origin
backbone_size4244
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
CRISPR
Other
E.coli
growth notes :
The HME63 strain is available from the from the Court Lab: https://redrecombineering.ncifcrf.gov/strains--plasmids.html For more information on Marraffini Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/marraffini/
growth strain :
Bacterial expression of Cas9 nuclease, tracrRNA and crRNA guide
growth temp :
pCas9 is resistant to chloramphenicol (25ug/ml).
origin :
37
pi :
alt_names
cloning
entrez_gene
genbank_ids
mutation
nametracr/Cas9
shRNA_sequence
size
species
tags
resistance markers :
1451
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA