This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-3myc-6His-EZH2 21D
catalog :
42664
citations: 2
Reference
Davies A, Nouruzi S, Ganguli D, Namekawa T, Thaper D, Linder S, et al. An androgen receptor switch underlies lineage infidelity in treatment-resistant prostate cancer. Nat Cell Biol. 2021;23:1023-1034 pubmed publisher
Cha T, Zhou B, Xia W, Wu Y, Yang C, Chen C, et al. Akt-mediated phosphorylation of EZH2 suppresses methylation of lysine 27 in histone H3. Science. 2005;310:306-10 pubmed
product information
Catalog Number :
42664
Product Name :
pcDNA3-3myc-6His-EZH2 21D
article :
doi10.1126/science.1118947
id6301
pubmed_id16224021
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size5446
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please note that there is an additional mutation in EZH2 ORF, Q3R. The mutation does not affect plasmid function.
origin :
37
pi :
alt_names
enhancer of zeste homolog 2
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5BamHI
promoter
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesENX-1, ENX1, EZH2b, KMT6, KMT6A, WVS, WVS2
geneEZH2
id2146
genbank_ids
mutationS21D
nameEZH2
shRNA_sequence
size2400
species
9606
Homo sapiens
tags
locationN terminal on insert
tag3Myc
locationN terminal on insert
tag6His
plasmid copy :
A. Chinnaiyan
resistance markers :
215
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA