This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGC479
catalog :
42601
citations: 1
product information
Catalog Number :
42601
Product Name :
pGC479
article :
| doi | 10.1242/dev.074047 |
| id | 6277 |
| pubmed_id | 22278922 |
bacterial resistance :
Ampicillin and Tetracycline
cloning :
| backbone | L4440 | |
| backbone_mutation | ||
| backbone_origin | ||
| backbone_size | 2790 | |
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth notes :
This plasmid can be kept in DH5alpha cells but does not work for RNAi in that strain. For RNAi, please transform DNA into the HT115 strain. Note: Addgene's quality control sequencing has found that the deposited clone contains a T to C mismatch with respect to the C. elegans N2 reference daf-15 sequence, which would cause a V797A change in the coded protein. According to the depositing lab, this point mutation does not affect RNAi feeding; however, its possible effects in other contexts are not known.
growth temp :
Grow on LB + Amp + Tet plates. For RNAi feeding, grow LB liquid culture with Amp only.
origin :
37
pi :
|
resistance markers :
| 212 |
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments