This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pNMT
catalog :
42578
citations: 5
Reference
Loats A, Carrera S, Fleming A, Roberts A, Sherrard A, Toska E, et al. Cholesterol is required for transcriptional repression by BASP1. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
Marklew C, Booth A, Beales P, Ciani B. Membrane remodelling by a lipidated endosomal sorting complex required for transport-III chimera, in vitro. Interface Focus. 2018;8:20180035 pubmed publisher
Sobajima T, Yoshimura S, Maeda T, Miyata H, Miyoshi E, Harada A. The Rab11-binding protein RELCH/KIAA1468 controls intracellular cholesterol distribution. J Cell Biol. 2018;217:1777-1796 pubmed publisher
Yang S, Lim S, Kiessling V, Kwon I, Tamm L. Site-specific fluorescent labeling to visualize membrane translocation of a myristoyl switch protein. Sci Rep. 2016;6:32866 pubmed publisher
Carlson L, Hurley J. In vitro reconstitution of the ordered assembly of the endosomal sorting complex required for transport at membrane-bound HIV-1 Gag clusters. Proc Natl Acad Sci U S A. 2012;109:16928-33 pubmed publisher
product information
Catalog Number :
42578
Product Name :
pNMT
article :
doi10.1073/pnas.1211759109
id6187
pubmed_id23027949
bacterial resistance :
Kanamycin
cloning :
backbonepRSF-1
backbone_mutation
backbone_originNovagen/EMD Millipore
backbone_size3600
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Used for production of myristylated HIV-1 Gag in E. coli.
origin :
37
pi :
alt_names
NMT
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NcoI
promoterT7
sequencing_primer_3T7term
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYLR195C, CDC72
geneNMT1
id850892
genbank_ids
M23726.1
mutationtwo silent mutations T690C and T942C
nameN-myristoyl tranferase
shRNA_sequence
size1368
species
4932
Saccharomyces cerevisiae
tags
resistance markers :
216
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA