This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pJK59-ATG18-GFP
catalog :
42529
citations: 1
Reference
Baskaran S, Ragusa M, Boura E, Hurley J. Two-site recognition of phosphatidylinositol 3-phosphate by PROPPINs in autophagy. Mol Cell. 2012;47:339-48 pubmed publisher
product information
Catalog Number :
42529
Product Name :
pJK59-ATG18-GFP
article :
doi10.1016/j.molcel.2012.05.027
id6263
pubmed_id22704557
bacterial resistance :
Ampicillin
cloning :
backbonepRS316
backbone_mutation
backbone_origin
backbone_size5756
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
Plasmid was constructed by amplifying Atg18 with 300bp 5' of the start site.
origin :
37
pi :
alt_names
svp1
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NotI
promoter
sequencing_primer_3
sequencing_primer_5internal primer
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYFR021W, AUT10, CVT18, NMR1, SVP1
geneATG18
id850577
genbank_ids
mutationnone
nameAtg18
shRNA_sequence
size1800
species
4932
Saccharomyces cerevisiae
tags
locationC terminal on backbone
tagGFP
plasmid copy :
the plasmid was obtained from Will Prinz
resistance markers :
216
tags :
Low Copy
terms :
URA3
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA