pLKO-shPKM2_4 | Addgene 42516 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLKO-shPKM2_4

catalog :

42516

citations: 5

Reference
Taylor S, Ramsamooj S, Liang R, Katti A, Pozovskiy R, VASAN N, et al. Dietary fructose improves intestinal cell survival and nutrient absorption. Nature. 2021;597:263-267 pubmed publisher
Jia Y, Li H, Wang Y, Wang J, Zhu J, Wei Y, et al. Crosstalk between hypoxia-sensing ULK1/2 and YAP-driven glycolysis fuels pancreatic ductal adenocarcinoma development. Int J Biol Sci. 2021;17:2772-2794 pubmed publisher
Morita M, Sato T, Nomura M, Sakamoto Y, Inoue Y, Tanaka R, et al. PKM1 Confers Metabolic Advantages and Promotes Cell-Autonomous Tumor Cell Growth. Cancer Cell. 2018;33:355-367.e7 pubmed publisher
Zhao J, Li J, Fan T, Hou S. Glycolytic reprogramming through PCK2 regulates tumor initiation of prostate cancer cells. Oncotarget. 2017;8:83602-83618 pubmed publisher
Anastasiou D, Poulogiannis G, Asara J, Boxer M, Jiang J, Shen M, et al. Inhibition of pyruvate kinase M2 by reactive oxygen species contributes to cellular antioxidant responses. Science. 2011;334:1278-83 pubmed publisher

product information

Catalog Number :

42516

Product Name :

pLKO-shPKM2_4

article :

doi	10.1126/science.1211485
id	6258
pubmed_id	22052977

bacterial resistance :

Ampicillin

cloning :

backbone

pLKO.1

backbone_mutation

backbone_origin

The RNAi consortium

backbone_size

7032

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Lentiviral

growth notes :

The depositing scientist notes: NM_011099.3 is PKM2 and NM_001253883.1 is PKM1, based on sequence homology of the respective translated products to the human PKM isoforms. The isoform annotations in the corresponding GeneBank entries are incorrect.

origin :

pi :

alt_names

cloning

entrez_gene

aliases	CTHBP, HEL-S-30, OIP3, PK3, PKM2, TCB, THBP1, p58
gene	PKM
id	5315

genbank_ids

NM_002654

NM_182471

mutation

name

shRNA-PKM2

shRNA_sequence

PKM2

size

species

9606

Homo sapiens