This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET28a-His-hPKM2
catalog :
42515
citations: 2
Reference
Macpherson J, Theisen A, Masino L, Fets L, Driscoll P, Encheva V, et al. Functional cross-talk between allosteric effects of activating and inhibiting ligands underlies PKM2 regulation. elife. 2019;8: pubmed publisher
Anastasiou D, Poulogiannis G, Asara J, Boxer M, Jiang J, Shen M, et al. Inhibition of pyruvate kinase M2 by reactive oxygen species contributes to cellular antioxidant responses. Science. 2011;334:1278-83 pubmed publisher
product information
Catalog Number :
42515
Product Name :
pET28a-His-hPKM2
article :
doi10.1126/science.1211485
id6258
pubmed_id22052977
bacterial resistance :
Kanamycin
cloning :
backbonepET28a
backbone_mutation
backbone_originNovagen
backbone_size5369
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Christofk, H. R., Vander Heiden, M. G., Wu, N., Asara, J. M., & Cantley, L. C. (2008). Pyruvate kinase M2 is a phosphotyrosine-binding protein. Nature, 452(7184), 181 186. doi:10.1038/nature06667
growth temp :
To produce protein, transform into BL21(DE3)pLysS cells.
origin :
37
pi :
alt_names
PKM2
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5NdeI
promoter
sequencing_primer_3
sequencing_primer_5T7 promoter primer
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCTHBP, HEL-S-30, OIP3, PK3, PKM2, TCB, THBP1, p58
genePKM
id5315
genbank_ids
mutation
namePyruvate Kinase M2
shRNA_sequence
size1600
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHis
plasmid copy :
Cloned by Ning Wu in Lew Cantley's Lab.
resistance markers :
1436
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA