pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

catalog :

42337

citations: 4

Reference
Stockman V, Ghamsari L, Lasso G, Honig B, Shapira S, Wang H. A High-Throughput Strategy for Dissecting Mammalian Genetic Interactions. PLoS ONE. 2016;11:e0167617 pubmed publisher
Dang Y, Jia G, Choi J, Ma H, Anaya E, Ye C, et al. Optimizing sgRNA structure to improve CRISPR-Cas9 knockout efficiency. Genome Biol. 2015;16:280 pubmed publisher
Ma H, Dang Y, Wu Y, Jia G, Anaya E, Zhang J, et al. A CRISPR-Based Screen Identifies Genes Essential for West-Nile-Virus-Induced Cell Death. Cell Rep. 2015;12:673-83 pubmed publisher
Cong L, Ran F, Cox D, Lin S, Barretto R, Habib N, et al. Multiplex genome engineering using CRISPR/Cas systems. Science. 2013;339:819-23 pubmed publisher

product information

Catalog Number :

42337

Product Name :

pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

article :

doi	10.1126/science.1231143
id	6225
pubmed_id	23287718

bacterial resistance :

Ampicillin

cloning :

backbone

pUC ori vector

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

CRISPR

growth notes :

Note, Addgene's quality control sequencing has found discrepancies with the depositor's full sequence. The depositing lab is aware of these discrepancies, and they are not thought to affect plasmid function. For more information on Zhang Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/zhang/

growth strain :

Dual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.

origin :

pi :

alt_names

SpCas9

hSpCas9

cloning

entrez_gene

genbank_ids

mutation

name

humanized S. pyogenes Cas9

shRNA_sequence

size

4272

species