Reference |
---|
Hwang Y, Seita Y, Blanco M, Sasaki K. CRISPR loss of function screening to identify genes involved in human primordial germ cell-like cell development. PLoS Genet. 2023;19:e1011080 pubmed publisher
|
Tsuchiya Y, Seki T, Kobayashi K, Komazawa Sakon S, Shichino S, Nishina T, et al. Fibroblast growth factor 18 stimulates the proliferation of hepatic stellate cells, thereby inducing liver fibrosis. Nat Commun. 2023;14:6304 pubmed publisher
|
Fuentes L, Marin Z, Tyson J, Baddeley D, Bewersdorf J. The nanoscale organization of reticulon 4 shapes local endoplasmic reticulum structure in situ. J Cell Biol. 2023;222: pubmed publisher
|
Didaskalou S, Efstathiou C, Galtsidis S, Kesisova I, Halavatyi A, Elmali T, et al. HURP localization in metaphase is the result of a multi-step process requiring its phosphorylation at Ser627 residue. Front Cell Dev Biol. 2023;11:981425 pubmed publisher
|
Zhou L, Zhang S, Li L, Yang G, Zeng L. Optimization of mammalian expression vector by cis-regulatory element combinations. Mol Genet Genomics. 2023;298:1121-1133 pubmed publisher
|
Sanchez Bri xf1 as A, Duran Ruiz C, Astola A, Arroyo M, Raposo F, Valle A, et al. ZNF330/NOA36 interacts with HSPA1 and HSPA8 and modulates cell cycle and proliferation in response to heat shock in HEK293 cells. Biol Direct. 2023;18:26 pubmed publisher
|
Bustos F, Pandian S, Haensgen H, Zhao J, Strouf H, Heidenreich M, et al. Removal of a genomic duplication by double-nicking CRISPR restores synaptic transmission and behavior in the MyosinVA mutant mouse Flailer. bioRxiv. 2023;: pubmed publisher
|
Davidson I, Barth R, Zaczek M, van der Torre J, Tang W, Nagasaka K, et al. CTCF is a DNA-tension-dependent barrier to cohesin-mediated loop extrusion. Nature. 2023;616:822-827 pubmed publisher
|
Ruminski K, Celis Gutierrez J, Jarmuzynski N, Maturin E, Audebert S, Malissen M, et al. Mapping the SLP76 interactome in T cells lacking each of the GRB2-family adaptors reveals molecular plasticity of the TCR signaling pathway. Front Immunol. 2023;14:1139123 pubmed publisher
|
Chung C, Verheijen B, Navapanich Z, McGann E, Shemtov S, Lai G, et al. Evolutionary conservation of the fidelity of transcription. Nat Commun. 2023;14:1547 pubmed publisher
|
Gyobu Motani S, Yabuta Y, Mizuta K, Katou Y, Okamoto I, Kawasaki M, et al. Induction of fetal meiotic oocytes from embryonic stem cells in cynomolgus monkeys. EMBO J. 2023;42:e112962 pubmed publisher
|
Matsuzaki M, Yokoyama M, Yoshizawa Y, Kaneko N, Naito H, Kobayashi H, et al. ADAMTS4 is involved in the production of the Alzheimer disease amyloid biomarker APP669-711. Mol Psychiatry. 2023;28:1802-1812 pubmed publisher
|
McMillan M, Gómez N, Hsieh C, Bekier M, Li X, Miguez R, et al. RNA methylation influences TDP43 binding and disease pathogenesis in models of amyotrophic lateral sclerosis and frontotemporal dementia. Mol Cell. 2023;: pubmed publisher
|
Jiang X, Isogai T, Chi J, Danuser G. Fine-grained, nonlinear registration of live cell movies reveals spatiotemporal organization of diffuse molecular processes. PLoS Comput Biol. 2022;18:e1009667 pubmed publisher
|
Bahrami Nejad Z, Zhang Z, Tholen S, Sharma S, Rabiee A, Zhao M, et al. Early enforcement of cell identity by a functional component of the terminally differentiated state. PLoS Biol. 2022;20:e3001900 pubmed publisher
|
Westermann L, Li Y, G xf6 cmen B, Niedermoser M, Rhein K, Jahn J, et al. Wildtype heterogeneity contributes to clonal variability in genome edited cells. Sci Rep. 2022;12:18211 pubmed publisher
|
Polasek Sedlackova H, Miller T, Krejci J, Rask M, Lukas J. Solving the MCM paradox by visualizing the scaffold of CMG helicase at active replisomes. Nat Commun. 2022;13:6090 pubmed publisher
|
Javaid N, Choi S. An Alternate Approach to Generate Induced Pluripotent Stem Cells with Precise CRISPR/Cas9 Tool. Stem Cells Int. 2022;2022:4537335 pubmed publisher
|
Millrine D, Cummings T, Matthews S, Peter J, Magnussen H, Lange S, et al. Human UFSP1 is an active protease that regulates UFM1 maturation and UFMylation. Cell Rep. 2022;40:111168 pubmed publisher
|
Patron M, Tarasenko D, Nolte H, Kroczek L, Ghosh M, Ohba Y, et al. Regulation of mitochondrial proteostasis by the proton gradient. EMBO J. 2022;41:e110476 pubmed publisher
|
Cypris O, Franzen J, Frobel J, Glück P, Kuo C, Schmitz S, et al. Hematopoietic differentiation persists in human iPSCs defective in de novo DNA methylation. BMC Biol. 2022;20:141 pubmed publisher
|
Lee J, Xu Y, Saidi L, Xu M, ZINSMAIER K, Ye Y. Abnormal triaging of misfolded proteins by adult neuronal ceroid lipofuscinosis-associated DNAJC5/CSPα mutants causes lipofuscin accumulation. Autophagy. 2022;:1-20 pubmed publisher
|
Song D, Takahashi G, Zheng Y, Matsuo Takasaki M, Li J, Takami M, et al. Retinoids rescue ceruloplasmin secretion and alleviate oxidative stress in Wilson's disease-specific hepatocytes. Hum Mol Genet. 2022;31:3652-3671 pubmed publisher
|
Günes Günsel G, Conlon T, Jeridi A, Kim R, Ertuz Z, Lang N, et al. The arginine methyltransferase PRMT7 promotes extravasation of monocytes resulting in tissue injury in COPD. Nat Commun. 2022;13:1303 pubmed publisher
|
de Toledo M, Fu X, Kluge F, Götz K, Schmitz S, Wanek P, et al. CRISPR/Cas9-engineered human ES cells harboring heterozygous and homozygous c-KIT knockout. Stem Cell Res. 2022;60:102732 pubmed publisher
|
Detilleux D, Raynaud P, Pradet Balade B, Helmlinger D. The TRRAP transcription cofactor represses interferon-stimulated genes in colorectal cancer cells. elife. 2022;11: pubmed publisher
|
Abel Y, Charron C, Virciglio C, Bourguignon Igel V, Quinternet M, Chagot M, et al. The interaction between RPAP3 and TRBP reveals a possible involvement of the HSP90/R2TP chaperone complex in the regulation of miRNA activity. Nucleic Acids Res. 2022;50:2172-2189 pubmed publisher
|
Maldotti M, Lauria A, Anselmi F, Molineris I, Tamburrini A, Meng G, et al. The acetyltransferase p300 is recruited in trans to multiple enhancer sites by lncSmad7. Nucleic Acids Res. 2022;50:2587-2602 pubmed publisher
|
Kakade P, Ojha H, Raimi O, Shaw A, Waddell A, Ault J, et al. Mapping of a N-terminal α-helix domain required for human PINK1 stabilization, Serine228 autophosphorylation and activation in cells. Open Biol. 2022;12:210264 pubmed publisher
|
Fenix A, Miyaoka Y, Bertero A, Blue S, Spindler M, Tan K, et al. Gain-of-function cardiomyopathic mutations in RBM20 rewire splicing regulation and re-distribute ribonucleoprotein granules within processing bodies. Nat Commun. 2021;12:6324 pubmed publisher
|
Chen B, Zhu G, Yan A, He J, Liu Y, Li L, et al. IGSF11 is required for pericentric heterochromatin dissociation during meiotic diplotene. PLoS Genet. 2021;17:e1009778 pubmed publisher
|
Šikrová D, Cadar V, Ariyurek Y, Laros J, Balog J, van der Maarel S. Adenine base editing of the DUX4 polyadenylation signal for targeted genetic therapy in facioscapulohumeral muscular dystrophy. Mol Ther Nucleic Acids. 2021;25:342-354 pubmed publisher
|
Mariani L, Guo X, Menezes N, Drozd A, Çakal S, Wang Q, et al. A TALE/HOX code unlocks WNT signalling response towards paraxial mesoderm. Nat Commun. 2021;12:5136 pubmed publisher
|
Dettmer R, Niwolik I, Mehmeti I, Jörns A, Naujok O. New hPSC SOX9 and INS Reporter Cell Lines Facilitate the Observation and Optimization of Differentiation into Insulin-Producing Cells. Stem Cell Rev Rep. 2021;17:2193-2209 pubmed publisher
|
Schimmel J, Muñoz Subirana N, Kool H, van Schendel R, Tijsterman M. Small tandem DNA duplications result from CST-guided Pol α-primase action at DNA break termini. Nat Commun. 2021;12:4843 pubmed publisher
|
Weißenbruch K, Grewe J, Hippler M, Fladung M, Tremmel M, Stricker K, et al. Distinct roles of nonmuscle myosin II isoforms for establishing tension and elasticity during cell morphodynamics. elife. 2021;10: pubmed publisher
|
Le T, Ainsworth J, Polo Rivera C, MacArtney T, Labib K. Reconstitution of human CMG helicase ubiquitylation by CUL2LRR1 and multiple E2 enzymes. Biochem J. 2021;: pubmed publisher
|
Sauer M, Was N, Ziegenhals T, Wang X, Hafner M, Becker M, et al. The miR-26 family regulates neural differentiation-associated microRNAs and mRNAs by directly targeting REST. J Cell Sci. 2021;134: pubmed publisher
|
Feng H, Li Q, Margolis R, Li P. Generation of a human induced pluripotent stem cell line JHUi003-A with homozygous mutation for spinocerebellar ataxia type 12 using genome editing. Stem Cell Res. 2021;53:102346 pubmed publisher
|
Li P, Margolis R. Use of single guided Cas9 nickase to facilitate precise and efficient genome editing in human iPSCs. Sci Rep. 2021;11:9865 pubmed publisher
|
Lu H, Liu J, Feng T, Guo Z, Yin Y, Gao F, et al. A HIT-trapping strategy for rapid generation of reversible and conditional alleles using a universal donor. Genome Res. 2021;31:900-909 pubmed publisher
|
Kaczmarczyk L, Reichenbach N, Blank N, Jonson M, Dittrich L, Petzold G, et al. Slc1a3-2A-CreERT2 mice reveal unique features of Bergmann glia and augment a growing collection of Cre drivers and effectors in the 129S4 genetic background. Sci Rep. 2021;11:5412 pubmed publisher
|
Thues C, Valadas J, Deaulmerie L, Geens A, Chouhan A, Duran Romaña R, et al. MAPRE2 mutations result in altered human cranial neural crest migration, underlying craniofacial malformations in CSC-KT syndrome. Sci Rep. 2021;11:4976 pubmed publisher
|
Kojima Y, Yamashiro C, Murase Y, Yabuta Y, Okamoto I, Iwatani C, et al. GATA transcription factors, SOX17 and TFAP2C, drive the human germ-cell specification program. Life Sci Alliance. 2021;4: pubmed publisher
|
Toledo M, Gatz M, Sontag S, Gleixner K, Eisenwort G, Feldberg K, et al. Nintedanib Targets KIT D816V Neoplastic Cells Derived from Induced Pluripotent Stem cells of Systemic Mastocytosis. Blood. 2020;: pubmed publisher
|
Gubas A, Karantanou C, Popovic D, Tascher G, Hoffmann M, Platzek A, et al. The endolysosomal adaptor PLEKHM1 is a direct target for both mTOR and MAPK pathways. FEBS Lett. 2021;595:864-880 pubmed publisher
|
Long Y, Cech T. Targeted mutagenesis in human iPSCs using CRISPR genome-editing tools. Methods. 2021;: pubmed publisher
|
Burda P, Bisio H, Marq J, Soldati Favre D, Heussler V. CRISPR/Cas9-Based Knockout of GNAQ Reveals Differences in Host Cell Signaling Necessary for Egress of Apicomplexan Parasites. mSphere. 2020;5: pubmed publisher
|
Liu N, Maresca M, van den Brand T, Braccioli L, Schijns M, Teunissen H, et al. WAPL maintains a cohesin loading cycle to preserve cell-type-specific distal gene regulation. Nat Genet. 2021;53:100-109 pubmed publisher
|
Mellis A, Misko A, Arjune S, Liang Y, Erdélyi K, Ditrói T, et al. The role of glutamate oxaloacetate transaminases in sulfite biosynthesis and H2S metabolism. Redox Biol. 2021;38:101800 pubmed publisher
|
Noda K, Kitagawa K, Miki T, Horiguchi M, Akama T, Taniguchi T, et al. A matricellular protein fibulin-4 is essential for the activation of lysyl oxidase. Sci Adv. 2020;6: pubmed publisher
|
Weltner J, Trokovic R. Reprogramming of Fibroblasts to Human iPSCs by CRISPR Activators. Methods Mol Biol. 2021;2239:175-198 pubmed publisher
|
Hwang Y, Suzuki S, Seita Y, Ito J, Sakata Y, Aso H, et al. Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cells. Nat Commun. 2020;11:5656 pubmed publisher
|
Narai T, Watase R, Nakayama Y, Kodani I, Inoue T, Kokura K. Establishment of human immortalized mesenchymal stem cells lines for the monitoring and analysis of osteogenic differentiation in living cells. Heliyon. 2020;6:e05398 pubmed publisher
|
Zhong A, Li M, Zhou T. Protocol for the Generation of Human Pluripotent Reporter Cell Lines Using CRISPR/Cas9. STAR Protoc. 2020;1: pubmed publisher
|
Jiao F, Li Z, He C, Xu W, Yang G, Liu T, et al. RACK7 recognizes H3.3G34R mutation to suppress expression of MHC class II complex components and their delivery pathway in pediatric glioblastoma. Sci Adv. 2020;6:eaba2113 pubmed publisher
|
Cypris O, Eipel M, Franzen J, Rösseler C, Tharmapalan V, Kuo C, et al. PRDM8 reveals aberrant DNA methylation in aging syndromes and is relevant for hematopoietic and neuronal differentiation. Clin Epigenetics. 2020;12:125 pubmed publisher
|
Hasan A, Dinh T, Le H, Mizuno Iijima S, Daitoku Y, Ishida M, et al. Characterization of a bicistronic knock-in reporter mouse model for investigating the role of CABLES2 in vivo. Exp Anim. 2021;70:22-30 pubmed publisher
|
Dettmer R, Naujok O. Design and Derivation of Multi-Reporter Pluripotent Stem Cell Lines via CRISPR/Cas9n-Mediated Homology-Directed Repair. Curr Protoc Stem Cell Biol. 2020;54:e116 pubmed publisher
|
León T, Rapoz D Silva T, Bertoli C, Rahman S, Magnussen M, Philip B, et al. EZH2-Deficient T-cell Acute Lymphoblastic Leukemia Is Sensitized to CHK1 Inhibition through Enhanced Replication Stress. Cancer Discov. 2020;10:998-1017 pubmed publisher
|
Lopez Guerrero A, Espinosa Bermejo N, Sanchez Lopez I, MacArtney T, Pascual Caro C, Orantos Aguilera Y, et al. RAC1-Dependent ORAI1 Translocation to the Leading Edge Supports Lamellipodia Formation and Directional Persistence. Sci Rep. 2020;10:6580 pubmed publisher
|
Vitre B, Taulet N, Guesdon A, Douanier A, Dosdane A, Cisneros M, et al. IFT proteins interact with HSET to promote supernumerary centrosome clustering in mitosis. EMBO Rep. 2020;21:e49234 pubmed publisher
|
Kojima Y, Machida Y, Palani S, Caulfield T, Radisky E, Kaufmann S, et al. FAM111A protects replication forks from protein obstacles via its trypsin-like domain. Nat Commun. 2020;11:1318 pubmed publisher
|
Liu R, Chen C, Li Y, Huang Q, Xue Y. ELL-associated factors EAF1/2 negatively regulate HIV-1 transcription through inhibition of Super Elongation Complex formation. Biochim Biophys Acta Gene Regul Mech. 2020;1863:194508 pubmed publisher
|
Wutz G, Ladurner R, St Hilaire B, Stocsits R, Nagasaka K, Pignard B, et al. ESCO1 and CTCF enable formation of long chromatin loops by protecting cohesinSTAG1 from WAPL. elife. 2020;9: pubmed publisher
|
Bozic M, van den Bekerom L, Milne B, Goodman N, Roberston L, Prescott A, et al. A conserved ATG2-GABARAP family interaction is critical for phagophore formation. EMBO Rep. 2020;21:e48412 pubmed publisher
|
Huo Z, Tu J, Lee D, Zhao R. Engineering Mutation Clones in Mammalian Cells with CRISPR/Cas9. Methods Mol Biol. 2020;2108:355-369 pubmed publisher
|
Ren T, Fan X, Wang M, Duan F, Wei C, Li R, et al. miR‑20b promotes growth of non‑small cell lung cancer through a positive feedback loop of the Wnt/β‑catenin signaling pathway. Int J Oncol. 2020;56:470-479 pubmed publisher
|
Morrison K, Manzano M, Chung K, Schipma M, Bartom E, Gottwein E. The Oncogenic Kaposi's Sarcoma-Associated Herpesvirus Encodes a Mimic of the Tumor-Suppressive miR-15/16 miRNA Family. Cell Rep. 2019;29:2961-2969.e6 pubmed publisher
|
Rodríguez Cruz P, Cossins J, Cheung J, Maxwell S, Jayawant S, Herbst R, et al. Congenital myasthenic syndrome due to mutations in MUSK suggests that the level of MuSK phosphorylation is crucial for governing synaptic structure. Hum Mutat. 2019;: pubmed publisher
|
Sakai Y, Nakamura T, Okamoto I, Gyobu Motani S, Ohta H, Yabuta Y, et al. Induction of the Germ-Cell Fate from Pluripotent Stem Cells in Cynomolgus Monkeys. Biol Reprod. 2019;: pubmed publisher
|
Liang J, Wang N, He J, Du J, Guo Y, Li L, et al. Induction of Sertoli-like cells from human fibroblasts by NR5A1 and GATA4. elife. 2019;8: pubmed publisher
|
Attwood K, Salsman J, Chung D, Mathavarajah S, Van Iderstine C, Dellaire G. PML isoform expression and DNA break location relative to PML nuclear bodies impacts the efficiency of homologous recombination. Biochem Cell Biol. 2019;: pubmed publisher
|
Malik N, Nirujogi R, Peltier J, MacArtney T, Wightman M, Prescott A, et al. Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44. Biochem J. 2019;476:3081-3107 pubmed publisher
|
Berndsen K, Lis P, Yeshaw W, Wawro P, Nirujogi R, Wightman M, et al. PPM1H phosphatase counteracts LRRK2 signaling by selectively dephosphorylating Rab proteins. elife. 2019;8: pubmed publisher
|
Duan F, Wang M, Cao Y, Dan Li -, Li R, Fan X, et al. MicroRNA-421 confers paclitaxel resistance by binding to the KEAP1 3'UTR and predicts poor survival in non-small cell lung cancer. Cell Death Dis. 2019;10:821 pubmed publisher
|
Ochs F, Karemore G, Miron E, Brown J, Sedlackova H, Rask M, et al. Stabilization of chromatin topology safeguards genome integrity. Nature. 2019;574:571-574 pubmed publisher
|
Strebinger D, Deluz C, Friman E, Govindan S, Alber A, Suter D. Endogenous fluctuations of OCT4 and SOX2 bias pluripotent cell fate decisions. Mol Syst Biol. 2019;15:e9002 pubmed publisher
|
Panier S, Maric M, Hewitt G, Mason Osann E, Gali H, Dai A, et al. SLX4IP Antagonizes Promiscuous BLM Activity during ALT Maintenance. Mol Cell. 2019;76:27-43.e11 pubmed publisher
|
Ritter N, Ali T, Kopitchinski N, Schuster P, Beisaw A, Hendrix D, et al. The lncRNA Locus Handsdown Regulates Cardiac Gene Programs and Is Essential for Early Mouse Development. Dev Cell. 2019;50:644-657.e8 pubmed publisher
|
Kizner V, Naujock M, Fischer S, Jäger S, Reich S, Schlotthauer I, et al. CRISPR/Cas9-mediated Knockout of the Neuropsychiatric Risk Gene KCTD13 Causes Developmental Deficits in Human Cortical Neurons Derived from Induced Pluripotent Stem Cells. Mol Neurobiol. 2020;57:616-634 pubmed publisher
|
Kaczmarczyk L, Bansal V, Rajput A, Rahman R, Krzyżak W, Degen J, et al. Tagger-A Swiss army knife for multiomics to dissect cell type-specific mechanisms of gene expression in mice. PLoS Biol. 2019;17:e3000374 pubmed publisher
|
Wood K, Rowlands C, Qureshi W, Thomas H, Buczek W, Briggs T, et al. Disease modeling of core pre-mRNA splicing factor haploinsufficiency. Hum Mol Genet. 2019;28:3704-3723 pubmed publisher
|
van Bemmel J, Galupa R, Gard C, Servant N, Picard C, Davies J, et al. The bipartite TAD organization of the X-inactivation center ensures opposing developmental regulation of Tsix and Xist. Nat Genet. 2019;: pubmed publisher
|
Bezares Calderón L, Berger J, Jasek S, Verasztó C, Mendes S, Gühmann M, et al. Neural circuitry of a polycystin-mediated hydrodynamic startle response for predator avoidance. elife. 2018;7: pubmed publisher
|
Barajas Mora E, Kleiman E, Xu J, Carrico N, Lu H, Oltz E, et al. A B-Cell-Specific Enhancer Orchestrates Nuclear Architecture to Generate a Diverse Antigen Receptor Repertoire. Mol Cell. 2019;73:48-60.e5 pubmed publisher
|
Hutchinson L, Bozatzi P, MacArtney T, Sapkota G. Generation of Endogenous BMP Transcriptional Reporter Cells Through CRISPR/Cas9 Genome Editing. Methods Mol Biol. 2019;1891:29-35 pubmed publisher
|
Tsang S, Guo Y, Chan L, Huang Y, Chow K. Generation and characterization of pathogenic Mab21l2(R51C) mouse model. Genesis. 2018;56:e23261 pubmed publisher
|
Sato T, Ogawa T. Generating Genetically Engineered Mice Using a Spermatogonial Stem Cell-Mediated Method. Methods Mol Biol. 2019;1874:87-98 pubmed publisher
|
Wang Y, Zhao J, Duan N, Liu W, Zhang Y, Zhou M, et al. Paired CRISPR/Cas9 Nickases Mediate Efficient Site-Specific Integration of F9 into rDNA Locus of Mouse ESCs. Int J Mol Sci. 2018;19: pubmed publisher
|
Tveriakhina L, Schuster Gossler K, Jarrett S, Andrawes M, Rohrbach M, Blacklow S, et al. The ectodomains determine ligand function in vivo and selectivity of DLL1 and DLL4 toward NOTCH1 and NOTCH2 in vitro. elife. 2018;7: pubmed publisher
|
Davies A, Itzhak D, Edgar J, Archuleta T, Hirst J, Jackson L, et al. AP-4 vesicles contribute to spatial control of autophagy via RUSC-dependent peripheral delivery of ATG9A. Nat Commun. 2018;9:3958 pubmed publisher
|
Tajan M, Hock A, Blagih J, Robertson N, Labuschagne C, Kruiswijk F, et al. A Role for p53 in the Adaptation to Glutamine Starvation through the Expression of SLC1A3. Cell Metab. 2018;28:721-736.e6 pubmed publisher
|
Taylor J, Fernandez D, Thornton S, Skeate J, Lühen K, Da Silva D, et al. Heterotetrameric annexin A2/S100A10 (A2t) is essential for oncogenic human papillomavirus trafficking and capsid disassembly, and protects virions from lysosomal degradation. Sci Rep. 2018;8:11642 pubmed publisher
|
Sevim H, Kocaefe Y, Onur M, Uckan Cetinkaya D, Gürpinar O. Bone marrow derived mesenchymal stem cells ameliorate inflammatory response in an in vitro model of familial hemophagocytic lymphohistiocytosis 2. Stem Cell Res Ther. 2018;9:198 pubmed publisher
|
Xu J, Li W, Hossen M, Jia Y, Li L, Huang Z. Optimized Plasmid Construction Strategy for Cas9. Cell Physiol Biochem. 2018;48:131-137 pubmed publisher
|
Gopalappa R, Song M, Chandrasekaran A, Das S, Haq S, Koh H, et al. Efficient genome editing by FACS enrichment of paired D10A Cas9 nickases coupled with fluorescent proteins. Arch Pharm Res. 2018;41:911-920 pubmed publisher
|
Koch B, Nijmeijer B, Kueblbeck M, Cai Y, Walther N, Ellenberg J. Generation and validation of homozygous fluorescent knock-in cells using CRISPR-Cas9 genome editing. Nat Protoc. 2018;13:1465-1487 pubmed publisher
|
Martyn I, Kanno T, Ruzo A, Siggia E, Brivanlou A. Self-organization of a human organizer by combined Wnt and Nodal signalling. Nature. 2018;558:132-135 pubmed publisher
|
Mir R, Tonelli F, Lis P, MacArtney T, Polinski N, Martinez T, et al. The Parkinson's disease VPS35[D620N] mutation enhances LRRK2-mediated Rab protein phosphorylation in mouse and human. Biochem J. 2018;475:1861-1883 pubmed publisher
|
Bütepage M, Preisinger C, von Kriegsheim A, Scheufen A, Lausberg E, Li J, et al. Nucleolar-nucleoplasmic shuttling of TARG1 and its control by DNA damage-induced poly-ADP-ribosylation and by nucleolar transcription. Sci Rep. 2018;8:6748 pubmed publisher
|
Slotta C, Storm J, Pfisterer N, Henkel E, Kleinwächter S, Pieper M, et al. IKK1/2 protect human cells from TNF-mediated RIPK1-dependent apoptosis in an NF-κB-independent manner. Biochim Biophys Acta Mol Cell Res. 2018;1865:1025-1033 pubmed publisher
|
Bahrami Nejad Z, Zhao M, Tholen S, Hunerdosse D, Tkach K, van Schie S, et al. A Transcriptional Circuit Filters Oscillating Circadian Hormonal Inputs to Regulate Fat Cell Differentiation. Cell Metab. 2018;27:854-868.e8 pubmed publisher
|
Gopalappa R, Suresh B, Ramakrishna S, Kim H. Paired D10A Cas9 nickases are sometimes more efficient than individual nucleases for gene disruption. Nucleic Acids Res. 2018;46:e71 pubmed publisher
|
Saez I, Koyuncu S, Gutiérrez García R, Dieterich C, Vilchez D. Insights into the ubiquitin-proteasome system of human embryonic stem cells. Sci Rep. 2018;8:4092 pubmed publisher
|
Pfeiffer M, Quaranta R, Piccini I, Fell J, Rao J, Röpke A, et al. Cardiogenic programming of human pluripotent stem cells by dose-controlled activation of EOMES. Nat Commun. 2018;9:440 pubmed publisher
|
Ruzo A, Croft G, Metzger J, Galgoczi S, Gerber L, Pellegrini C, et al. Chromosomal instability during neurogenesis in Huntington's disease. Development. 2018;145: pubmed publisher
|
Oikawa T, Otsuka Y, Onodera Y, Horikawa M, Handa H, Hashimoto S, et al. Necessity of p53-binding to the CDH1 locus for its expression defines two epithelial cell types differing in their integrity. Sci Rep. 2018;8:1595 pubmed publisher
|
Quaranta R, Fell J, Rühle F, Rao J, Piccini I, Arauzo Bravo M, et al. Revised roles of ISL1 in a hES cell-based model of human heart chamber specification. elife. 2018;7: pubmed publisher
|
Zunke F, Moise A, Belur N, Gelyana E, Stojkovska I, Dzaferbegovic H, et al. Reversible Conformational Conversion of α-Synuclein into Toxic Assemblies by Glucosylceramide. Neuron. 2018;97:92-107.e10 pubmed publisher
|
Adikusuma F, Pfitzner C, Thomas P. Versatile single-step-assembly CRISPR/Cas9 vectors for dual gRNA expression. PLoS ONE. 2017;12:e0187236 pubmed publisher
|
Taulet N, Vitre B, Anguille C, Douanier A, Rocancourt M, Taschner M, et al. IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning. Nat Commun. 2017;8:1928 pubmed publisher
|
Jacobs E, Warrier S, Volders P, D haene E, Van Lombergen E, Vantomme L, et al. CRISPR/Cas9-mediated genome editing in naïve human embryonic stem cells. Sci Rep. 2017;7:16650 pubmed publisher
|
Wu W, Tsai Y, Justus S, Cho G, Sengillo J, Xu Y, et al. CRISPR Repair Reveals Causative Mutation in a Preclinical Model of Retinitis Pigmentosa: A Brief Methodology. Methods Mol Biol. 2018;1715:191-205 pubmed publisher
|
Honda I, Araki K, Honda S, Soeda F, Shin M, Misumi S, et al. Deletion of GIRK2 subunit containing GIRK channels of neurons expressing dopamine transporter decrease immobility time on forced swimming in mice. Neurosci Lett. 2018;665:140-146 pubmed publisher
|
Malik N, MacArtney T, Hornberger A, Anderson K, Tovell H, Prescott A, et al. Mechanism of activation of SGK3 by growth factors via the Class 1 and Class 3 PI3Ks. Biochem J. 2018;475:117-135 pubmed publisher
|
Schimmel J, Kool H, van Schendel R, Tijsterman M. Mutational signatures of non-homologous and polymerase theta-mediated end-joining in embryonic stem cells. EMBO J. 2017;36:3634-3649 pubmed publisher
|
Kulcsár P, Tálas A, Huszár K, Ligeti Z, Tóth E, Weinhardt N, et al. Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage. Genome Biol. 2017;18:190 pubmed publisher
|
Kojima Y, Sasaki K, Yokobayashi S, Sakai Y, Nakamura T, Yabuta Y, et al. Evolutionarily Distinctive Transcriptional and Signaling Programs Drive Human Germ Cell Lineage Specification from Pluripotent Stem Cells. Cell Stem Cell. 2017;21:517-532.e5 pubmed publisher
|
Piccini I, Fehrmann E, Frank S, Müller F, Greber B, Seebohm G. Adrenergic Stress Protection of Human iPS Cell-Derived Cardiomyocytes by Fast Kv7.1 Recycling. Front Physiol. 2017;8:705 pubmed publisher
|
Miyauchi H, Ohta H, Nagaoka S, Nakaki F, Sasaki K, Hayashi K, et al. Bone morphogenetic protein and retinoic acid synergistically specify female germ-cell fate in mice. EMBO J. 2017;36:3100-3119 pubmed publisher
|
Mages C, Wintsche A, Bernhart S, Müller G. The DREAM complex through its subunit Lin37 cooperates with Rb to initiate quiescence. elife. 2017;6: pubmed publisher
|
Corkery D, Clarke L, Gebremeskel S, Salsman J, Pinder J, Le Page C, et al. Loss of PRP4K drives anoikis resistance in part by dysregulation of epidermal growth factor receptor endosomal trafficking. Oncogene. 2018;37:174-184 pubmed publisher
|
Lopez Guerrero A, Pascual Caro C, Martín Romero F, Pozo Guisado E. Store-operated calcium entry is dispensable for the activation of ERK1/2 pathway in prostate cancer cells. Cell Signal. 2017;40:44-52 pubmed publisher
|
Schmuckli Maurer J, Reber V, Wacker R, Bindschedler A, Zakher A, Heussler V. Inverted recruitment of autophagy proteins to the Plasmodium berghei parasitophorous vacuole membrane. PLoS ONE. 2017;12:e0183797 pubmed publisher
|
Stanley W, Trivedi P, Sutherland A, Thomas H, Gurzov E. Differential regulation of pro-inflammatory cytokine signalling by protein tyrosine phosphatases in pancreatic β-cells. J Mol Endocrinol. 2017;59:325-337 pubmed publisher
|
Hanssen L, Kassouf M, Oudelaar A, Biggs D, Preece C, Downes D, et al. Tissue-specific CTCF-cohesin-mediated chromatin architecture delimits enhancer interactions and function in vivo. Nat Cell Biol. 2017;19:952-961 pubmed publisher
|
Marczenke M, Piccini I, Mengarelli I, Fell J, Röpke A, Seebohm G, et al. Cardiac Subtype-Specific Modeling of Kv1.5 Ion Channel Deficiency Using Human Pluripotent Stem Cells. Front Physiol. 2017;8:469 pubmed publisher
|
Tálas A, Kulcsár P, Weinhardt N, Borsy A, Tóth E, Szebényi K, et al. A convenient method to pre-screen candidate guide RNAs for CRISPR/Cas9 gene editing by NHEJ-mediated integration of a 'self-cleaving' GFP-expression plasmid. DNA Res. 2017;24:609-621 pubmed publisher
|
Marczenke M, Fell J, Piccini I, Röpke A, Seebohm G, Greber B. Generation and cardiac subtype-specific differentiation of PITX2-deficient human iPS cell lines for exploring familial atrial fibrillation. Stem Cell Res. 2017;21:26-28 pubmed publisher
|
RodrÃguez Hernández G, Hauer J, MartÃn Lorenzo A, Schäfer D, Bartenhagen C, GarcÃa RamÃrez I, et al. Infection Exposure Promotes ETV6-RUNX1 Precursor B-cell Leukemia via Impaired H3K4 Demethylases. Cancer Res. 2017;77:4365-4377 pubmed publisher
|
Fafilek B, Hampl M, Ricankova N, Vesela I, Balek L, Kunova Bosakova M, et al. Statins do not inhibit the FGFR signaling in chondrocytes. Osteoarthritis Cartilage. 2017;25:1522-1530 pubmed publisher
|
Wacker R, Eickel N, Schmuckli Maurer J, Annoura T, Niklaus L, Khan S, et al. LC3-association with the parasitophorous vacuole membrane of Plasmodium berghei liver stages follows a noncanonical autophagy pathway. Cell Microbiol. 2017;19: pubmed publisher
|
Nora E, Goloborodko A, Valton A, Gibcus J, Uebersohn A, Abdennur N, et al. Targeted Degradation of CTCF Decouples Local Insulation of Chromosome Domains from Genomic Compartmentalization. Cell. 2017;169:930-944.e22 pubmed publisher
|
Lopez Guerrero A, Tomas Martin P, Pascual Caro C, MacArtney T, Rojas Fernandez A, Ball G, et al. Regulation of membrane ruffling by polarized STIM1 and ORAI1 in cortactin-rich domains. Sci Rep. 2017;7:383 pubmed publisher
|
Li Z, Bi Y, Xiao H, Sun L, Ren Y, Li Y, et al. CRISPR-Cas9 system-driven site-specific selection pressure on Herpes simplex virus genomes. Virus Res. 2018;244:286-295 pubmed publisher
|
Wen Y, Liao G, PRITCHARD T, Zhao T, Connelly J, Pruett Miller S, et al. A stable but reversible integrated surrogate reporter for assaying CRISPR/Cas9-stimulated homology-directed repair. J Biol Chem. 2017;292:6148-6162 pubmed publisher
|
Lv J, Zhou G, Chen X, Chen H, Wu K, Xiang L, et al. Targeted RP9 ablation and mutagenesis in mouse photoreceptor cells by CRISPR-Cas9. Sci Rep. 2017;7:43062 pubmed publisher
|
Chen P, Bendris N, Hsiao Y, Reis C, Mettlen M, Chen H, et al. Crosstalk between CLCb/Dyn1-Mediated Adaptive Clathrin-Mediated Endocytosis and Epidermal Growth Factor Receptor Signaling Increases Metastasis. Dev Cell. 2017;40:278-288.e5 pubmed publisher
|
El Fatimy R, Subramanian S, Uhlmann E, Krichevsky A. Genome Editing Reveals Glioblastoma Addiction to MicroRNA-10b. Mol Ther. 2017;25:368-378 pubmed publisher
|
Sontag S, Förster M, Qin J, Wanek P, Mitzka S, Schüler H, et al. Modelling IRF8 Deficient Human Hematopoiesis and Dendritic Cell Development with Engineered iPS Cells. Stem Cells. 2017;35:898-908 pubmed publisher
|
Basiri M, Behmanesh M, Tahamtani Y, Khalooghi K, Moradmand A, Baharvand H. The Convenience of Single Homology Arm Donor DNA and CRISPR/Cas9-Nickase for Targeted Insertion of Long DNA Fragment. Cell J. 2017;18:532-539 pubmed
|
Van Nostrand E, Gelboin Burkhart C, Wang R, Pratt G, Blue S, Yeo G. CRISPR/Cas9-mediated integration enables TAG-eCLIP of endogenously tagged RNA binding proteins. Methods. 2017;118-119:50-59 pubmed publisher
|
Perelman S, Abrams M, Eitson J, Chen D, Jimenez A, Mettlen M, et al. Cell-Based Screen Identifies Human Interferon-Stimulated Regulators of Listeria monocytogenes Infection. PLoS Pathog. 2016;12:e1006102 pubmed publisher
|
Boehm E, Zornoza M, Jourdain A, Delmiro Magdalena A, Garcia Consuegra I, Torres Merino R, et al. Role of FAST Kinase Domains 3 (FASTKD3) in Post-transcriptional Regulation of Mitochondrial Gene Expression. J Biol Chem. 2016;291:25877-25887 pubmed
|
Fulcher L, MacArtney T, Bozatzi P, Hornberger A, Rojas Fernandez A, Sapkota G. An affinity-directed protein missile system for targeted proteolysis. Open Biol. 2016;6: pubmed
|
Roncagalli R, Cucchetti M, Jarmuzynski N, Gregoire C, Bergot E, Audebert S, et al. The scaffolding function of the RLTPR protein explains its essential role for CD28 co-stimulation in mouse and human T cells. J Exp Med. 2016;213:2437-2457 pubmed
|
Woo D, Chen Q, Yang T, Glineburg M, Hoge C, Leu N, et al. Enhancing a Wnt-Telomere Feedback Loop Restores Intestinal Stem Cell Function in a Human Organotypic Model of Dyskeratosis Congenita. Cell Stem Cell. 2016;19:397-405 pubmed publisher
|
Santos D, Kiskinis E, Eggan K, Merkle F. Comprehensive Protocols for CRISPR/Cas9-based Gene Editing in Human Pluripotent Stem Cells. Curr Protoc Stem Cell Biol. 2016;38:5B.6.1-5B.6.60 pubmed publisher
|
Golias T, Papandreou I, Sun R, Kumar B, Brown N, Swanson B, et al. Hypoxic repression of pyruvate dehydrogenase activity is necessary for metabolic reprogramming and growth of model tumours. Sci Rep. 2016;6:31146 pubmed publisher
|
Ding X, Ray Chaudhuri A, Callen E, Pang Y, Biswas K, Klarmann K, et al. Synthetic viability by BRCA2 and PARP1/ARTD1 deficiencies. Nat Commun. 2016;7:12425 pubmed publisher
|
Li C, Ding L, Sun C, Wu L, Zhou D, Pawlik K, et al. Novel HDAd/EBV Reprogramming Vector and Highly Efficient Ad/CRISPR-Cas Sickle Cell Disease Gene Correction. Sci Rep. 2016;6:30422 pubmed publisher
|
Heintze J, Costa J, Weber M, Ketteler R. Ribose 5-phosphate isomerase inhibits LC3 processing and basal autophagy. Cell Signal. 2016;28:1380-8 pubmed publisher
|
Zhang J, Huang K, O Neill K, Pang X, Luo X. Bax/Bak activation in the absence of Bid, Bim, Puma, and p53. Cell Death Dis. 2016;7:e2266 pubmed publisher
|
Park A, Yun T, Vigant F, Pernet O, Won S, Dawes B, et al. Nipah Virus C Protein Recruits Tsg101 to Promote the Efficient Release of Virus in an ESCRT-Dependent Pathway. PLoS Pathog. 2016;12:e1005659 pubmed publisher
|
O Neill K, Huang K, Zhang J, Chen Y, Luo X. Inactivation of prosurvival Bcl-2 proteins activates Bax/Bak through the outer mitochondrial membrane. Genes Dev. 2016;30:973-88 pubmed publisher
|
Yang D, Scavuzzo M, Chmielowiec J, Sharp R, Bajić A, Borowiak M. Enrichment of G2/M cell cycle phase in human pluripotent stem cells enhances HDR-mediated gene repair with customizable endonucleases. Sci Rep. 2016;6:21264 pubmed publisher
|
Mianné J, Chessum L, Kumar S, Aguilar C, Codner G, Hutchison M, et al. Correction of the auditory phenotype in C57BL/6N mice via CRISPR/Cas9-mediated homology directed repair. Genome Med. 2016;8:16 pubmed publisher
|
Wu J, Hunt S, Xue H, Liu Y, Darabi R. Generation and Characterization of a MYF5 Reporter Human iPS Cell Line Using CRISPR/Cas9 Mediated Homologous Recombination. Sci Rep. 2016;6:18759 pubmed publisher
|
Logan C, Cossins J, RodrÃguez Cruz P, Parry D, Maxwell S, MartÃnez MartÃnez P, et al. Congenital Myasthenic Syndrome Type 19 Is Caused by Mutations in COL13A1, Encoding the Atypical Non-fibrillar Collagen Type XIII α1 Chain. Am J Hum Genet. 2015;97:878-85 pubmed publisher
|
Lai Y, Kondapalli C, Lehneck R, Procter J, Dill B, Woodroof H, et al. Phosphoproteomic screening identifies Rab GTPases as novel downstream targets of PINK1. EMBO J. 2015;34:2840-61 pubmed publisher
|
Pinder J, Salsman J, Dellaire G. Nuclear domain 'knock-in' screen for the evaluation and identification of small molecule enhancers of CRISPR-based genome editing. Nucleic Acids Res. 2015;43:9379-92 pubmed publisher
|
Haraguchi M, Sato M, Ozawa M. CRISPR/Cas9n-Mediated Deletion of the Snail 1Gene (SNAI1) Reveals Its Role in Regulating Cell Morphology, Cell-Cell Interactions, and Gene Expression in Ovarian Cancer (RMG-1) Cells. PLoS ONE. 2015;10:e0132260 pubmed publisher
|
Flynn R, Grundmann A, Renz P, Hänseler W, James W, Cowley S, et al. CRISPR-mediated genotypic and phenotypic correction of a chronic granulomatous disease mutation in human iPS cells. Exp Hematol. 2015;43:838-848.e3 pubmed publisher
|
Ochiai H, Sugawara T, Yamamoto T. Simultaneous live imaging of the transcription and nuclear position of specific genes. Nucleic Acids Res. 2015;43:e127 pubmed publisher
|
Merkle F, Neuhausser W, Santos D, Valen E, Gagnon J, Maas K, et al. Efficient CRISPR-Cas9-mediated generation of knockin human pluripotent stem cells lacking undesired mutations at the targeted locus. Cell Rep. 2015;11:875-883 pubmed publisher
|
Parikh B, Beckman D, Patel S, White J, Yokoyama W. Detailed phenotypic and molecular analyses of genetically modified mice generated by CRISPR-Cas9-mediated editing. PLoS ONE. 2015;10:e0116484 pubmed publisher
|
Frock R, Hu J, Meyers R, Ho Y, Kii E, Alt F. Genome-wide detection of DNA double-stranded breaks induced by engineered nucleases. Nat Biotechnol. 2015;33:179-86 pubmed publisher
|
Munoz M, Yanez D, Stark J. An RNF168 fragment defective for focal accumulation at DNA damage is proficient for inhibition of homologous recombination in BRCA1 deficient cells. Nucleic Acids Res. 2014;42:7720-33 pubmed publisher
|
Wu X, Scott D, Kriz A, Chiu A, Hsu P, Dadon D, et al. Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells. Nat Biotechnol. 2014;32:670-6 pubmed publisher
|
Cheng A, Wang H, Yang H, Shi L, Katz Y, Theunissen T, et al. Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cell Res. 2013;23:1163-71 pubmed publisher
|
Cong L, Ran F, Cox D, Lin S, Barretto R, Habib N, et al. Multiplex genome engineering using CRISPR/Cas systems. Science. 2013;339:819-23 pubmed publisher
|