pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro

catalog :

42333

citations: 4

Reference
Xu J, Li W, Hossen M, Jia Y, Li L, Huang Z. Optimized Plasmid Construction Strategy for Cas9. Cell Physiol Biochem. 2018;48:131-137 pubmed publisher
Kurz C, Charroux B, Chaduli D, Viallat Lieutaud A, Royet J. Peptidoglycan sensing by octopaminergic neurons modulates Drosophila oviposition. elife. 2017;6: pubmed publisher
Maure J, Moser S, Jaffray E, F Alpi A, Hay R. Loss of ubiquitin E2 Ube2w rescues hypersensitivity of Rnf4 mutant cells to DNA damage. Sci Rep. 2016;6:26178 pubmed publisher
Cong L, Ran F, Cox D, Lin S, Barretto R, Habib N, et al. Multiplex genome engineering using CRISPR/Cas systems. Science. 2013;339:819-23 pubmed publisher

product information

Catalog Number :

42333

Product Name :

pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro

article :

doi	10.1126/science.1231143
id	6225
pubmed_id	23287718

bacterial resistance :

Ampicillin

cloning :

backbone

pUC ori vector

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

CRISPR

growth notes :

Note, Addgene's quality control sequencing has found a few discrepancies with the depositor's full sequence. The depositing lab is aware of these discrepancies, and they are not thought to affect plasmid function. For more information on Zhang Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/zhang/

growth strain :

This plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA.

origin :

pi :

alt_names

SpCas9n (D10A)

hSpCas9n (D10A)

cloning

entrez_gene

genbank_ids

mutation

D10A nickase-converting mutation

name

humanized S. pyogenes Cas9 (D10A) nickase

shRNA_sequence

size

4272

species