pEGFP VAMP7 (1-120) | Addgene 42317 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pEGFP VAMP7 (1-120)

catalog :

42317

citations: 1

Reference
Martinez Arca S, Alberts P, Zahraoui A, Louvard D, Galli T. Role of tetanus neurotoxin insensitive vesicle-associated membrane protein (TI-VAMP) in vesicular transport mediating neurite outgrowth. J Cell Biol. 2000;149:889-900 pubmed

product information

Catalog Number :

42317

Product Name :

pEGFP VAMP7 (1-120)

article :

doi	10.1083/jcb.149.4.889
id	6243
pubmed_id	10811829

bacterial resistance :

Kanamycin

cloning :

backbone

pEGFP-C3

backbone_mutation

backbone_origin

Clontech

backbone_size

4700

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Alternate plasmid name: NH2-terminal domain-TIVAMP (Nter-TIVAMP, from M to N120) For production of NH2-terminal GFP fusion proteins, the distinct cDNAs were cloned into the pEGFP-C3 vector (CLONTECH Laboratories, Inc.). Full-length TI-VAMP (TIVAMP), NH2-terminal domain-TIVAMP (Nter-TIVAMP, from M to N120), cytosolic domain-TIVAMP (Cyt-TIVAMP, from M to K188), and NH2-terminal domain-TIVAMP ( Nter-TIVAMP, from M102 to the end), were obtained by PCR using standard procedures and the following sets of oligonucleotides: 5 -ATGGCGATTCTTTTTGCTGTTGTTGCC-3 and 5 -CTATTTCTTCACACAGCTTGGCCATGT-3 for TIVAMP; 5 -ATGGCGATTCTTTTTGCTGTTGTTGCC-3 and 5 -CTTATTCTCAGAGTGATGCTTCAG-CTG-3 for Nter-TIVAMP; 5 -ATGGCGATTCTTTTTGCTGTTGTTGCC-3 and 5 -ATCCTACTTGAGGTTCTTCATACACATGGCTC for Cyt-TIVAMP; 5 -ATGAATAGCGAGTTCTCAAGTGTCTTA-3 and 5 -CTATTTCTTCACACAGCTTGGCCATGT-3 for Nter-TIVAMP.

origin :

pi :

alt_names

VAMP7

vesicle-associated membrane protein 7

TI-VAMP/Synaptobrevin-like (Sybl 1)

cloning

clone_method	Restriction Enzyme
cloning_site_3	XbaI
cloning_site_5	KpnI
promoter	CMV
sequencing_primer_3	SV40-pA-R
sequencing_primer_5	EGFP-C; CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	SYBL1, TI-VAMP, TIVAMP, VAMP-7
gene	VAMP7
id	6845

genbank_ids

NM_005638.5

NP_005629.1

mutation

120-220

name

VAMP7 Longin domain (1-120)

shRNA_sequence

size

species

9606

Homo sapiens