pEGFP VAMP7 (1-220) | Addgene 42316 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pEGFP VAMP7 (1-220)

catalog :

42316

citations: 7

Reference
Elfmark L, Wenzel E, Wang L, Pedersen N, Stenmark H, Raiborg C. Protrudin-mediated ER-endosome contact sites promote phagocytosis. Cell Mol Life Sci. 2023;80:216 pubmed publisher
Mao M, Chang C, Pickar Oliver A, Cervia L, Wang L, Ji J, et al. Redirecting Vesicular Transport to Improve Nonviral Delivery of Molecular Cargo. Adv Biosyst. 2020;4:e2000059 pubmed publisher
Chou H, Lee Y, Lin Y, Wen J, Peng W, Hsieh P, et al. PTPN9-mediated dephosphorylation of VTI1B promotes ATG16L1 precursor fusion and autophagosome formation. Autophagy. 2020;:1-16 pubmed publisher
Chitirala P, Ravichandran K, Galgano D, Sleiman M, Krause E, Bryceson Y, et al. Cytotoxic Granule Exocytosis From Human Cytotoxic T Lymphocytes Is Mediated by VAMP7. Front Immunol. 2019;10:1855 pubmed publisher
Martín Granado V, Ortiz Rivero S, Carmona R, Gutiérrez Herrero S, Barrera M, San Segundo L, et al. C3G promotes a selective release of angiogenic factors from activated mouse platelets to regulate angiogenesis and tumor metastasis. Oncotarget. 2017;8:110994-111011 pubmed publisher
Lee J, Takahama S, Zhang G, Tomarev S, Ye Y. Unconventional secretion of misfolded proteins promotes adaptation to proteasome dysfunction in mammalian cells. Nat Cell Biol. 2016;18:765-76 pubmed publisher
Martinez Arca S, Alberts P, Zahraoui A, Louvard D, Galli T. Role of tetanus neurotoxin insensitive vesicle-associated membrane protein (TI-VAMP) in vesicular transport mediating neurite outgrowth. J Cell Biol. 2000;149:889-900 pubmed

product information

Catalog Number :

42316

Product Name :

pEGFP VAMP7 (1-220)

article :

doi	10.1083/jcb.149.4.889
id	6243
pubmed_id	10811829

bacterial resistance :

Kanamycin

cloning :

backbone

pEGFP-C3

backbone_mutation

backbone_origin

Clontech

backbone_size

4700

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Alternate plasmid name: Full-length TI-VAMP (TIVAMP) For production of NH2-terminal GFP fusion proteins, the distinct cDNAs were cloned into the pEGFP-C3 vector (CLONTECH Laboratories, Inc.). Full-length TI-VAMP (TIVAMP), NH2-terminal domain-TIVAMP (Nter-TIVAMP, from M to N120), cytosolic domain-TIVAMP (Cyt-TIVAMP, from M to K188), and NH2-terminal domain-TIVAMP ( Nter-TIVAMP, from M102 to the end), were obtained by PCR using standard procedures and the following sets of oligonucleotides: 5 -ATGGCGATTCTTTTTGCTGTTGTTGCC-3 and 5 -CTATTTCTTCACACAGCTTGGCCATGT-3 for TIVAMP; 5 -ATGGCGATTCTTTTTGCTGTTGTTGCC-3 and 5 -CTTATTCTCAGAGTGATGCTTCAG-CTG-3 for Nter-TIVAMP; 5 -ATGGCGATTCTTTTTGCTGTTGTTGCC-3 and 5 -ATCCTACTTGAGGTTCTTCATACACATGGCTC for Cyt-TIVAMP; 5 -ATGAATAGCGAGTTCTCAAGTGTCTTA-3 and 5 -CTATTTCTTCACACAGCTTGGCCATGT-3 for Nter-TIVAMP.

origin :

pi :

alt_names

VAMP7

vesicle-associated membrane protein 7

TI-VAMP/Synaptobrevin-like (Sybl 1)

cloning

clone_method	Restriction Enzyme
cloning_site_3	BamHI
cloning_site_5	EcoRI
promoter	CMV
sequencing_primer_3	SV40-pA-R
sequencing_primer_5	EGFP-C; CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	SYBL1, TI-VAMP, TIVAMP, VAMP-7
gene	VAMP7
id	6845

genbank_ids

NM_005638.5

NP_005629.1

mutation

name

VAMP7 (1-220)

shRNA_sequence

size

species

9606

Homo sapiens