This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGEX VAMP7 (1-188)
catalog :
42315
citations: 2
Reference
Kruse T, Benz C, Garvanska D, Lindqvist R, Mihalic F, Coscia F, et al. Large scale discovery of coronavirus-host factor protein interaction motifs reveals SARS-CoV-2 specific mechanisms and vulnerabilities. Nat Commun. 2021;12:6761 pubmed publisher
Martinez Arca S, Alberts P, Zahraoui A, Louvard D, Galli T. Role of tetanus neurotoxin insensitive vesicle-associated membrane protein (TI-VAMP) in vesicular transport mediating neurite outgrowth. J Cell Biol. 2000;149:889-900 pubmed
product information
Catalog Number :
42315
Product Name :
pGEX VAMP7 (1-188)
article :
doi10.1083/jcb.149.4.889
id6243
pubmed_id10811829
bacterial resistance :
Ampicillin
cloning :
backbonepGEX 4T
backbone_mutation
backbone_originAmersham
backbone_size5000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Alternate plasmid name: cytosolic domain-TIVAMP (Cyt-TIVAMP) Although listed as 1-188 in the publication, this construct contains residues 1-181 and the truncation does not affect plasmid function.
origin :
37
pi :
alt_names
VAMP7
vesicle-associated membrane protein 7
TI-VAMP/Synaptobrevin-like (Sybl 1)
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5EcoRI
promoterPtac
sequencing_primer_3pGEX3
sequencing_primer_5pGEX5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesSYBL1, TI-VAMP, TIVAMP, VAMP-7
geneVAMP7
id6845
genbank_ids
NM_005638.5
NP_005629.1
mutation 181-220
nameVAMP7 Cytoplasmic domain (1-181)
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagGST
resistance markers :
1431
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA