This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEGFP VAMP2
catalog :
42308
citations: 8
Reference
Stévenin V, Chang Y, Le Toquin Y, Duchateau M, Gianetto Q, Luk C, et al. Dynamic Growth and Shrinkage of the Salmonella-Containing Vacuole Determines the Intracellular Pathogen Niche. Cell Rep. 2019;29:3958-3973.e7 pubmed publisher
Ahmed S, Nishida Fukuda H, Li Y, McDonald W, Gradinaru C, Macara I. Exocyst dynamics during vesicle tethering and fusion. Nat Commun. 2018;9:5140 pubmed publisher
Dirk B, End C, Pawlak E, Van Nynatten L, Jacob R, Heit B, et al. PACS-1 and adaptor protein-1 mediate ACTH trafficking to the regulated secretory pathway. Biochem Biophys Res Commun. 2018;507:519-525 pubmed publisher
Shinoda H, Ma Y, Nakashima R, Sakurai K, Matsuda T, Nagai T. Acid-Tolerant Monomeric GFP from Olindias formosa. Cell Chem Biol. 2018;25:330-338.e7 pubmed publisher
Vashi N, Andrabi S, Ghanwat S, Suar M, Kumar D. Ca2+-dependent Focal Exocytosis of Golgi-derived Vesicles Helps Phagocytic Uptake in Macrophages. J Biol Chem. 2017;292:5144-5165 pubmed publisher
Lee J, Takahama S, Zhang G, Tomarev S, Ye Y. Unconventional secretion of misfolded proteins promotes adaptation to proteasome dysfunction in mammalian cells. Nat Cell Biol. 2016;18:765-76 pubmed publisher
Deng H, Shi Y, Yang Y, Ahmeti K, Miller N, Huang C, et al. Identification of TMEM230 mutations in familial Parkinson's disease. Nat Genet. 2016;48:733-9 pubmed publisher
Martinez Arca S, Rudge R, Vacca M, Raposo G, Camonis J, Proux Gillardeaux V, et al. A dual mechanism controlling the localization and function of exocytic v-SNAREs. Proc Natl Acad Sci U S A. 2003;100:9011-6 pubmed
product information
Catalog Number :
42308
Product Name :
pEGFP VAMP2
article :
doi10.1073/pnas.1431910100
id6238
pubmed_id12853575
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C3
backbone_mutation
backbone_originClontech
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The V8A & T116S substitutions found in the Addgene sequence correspond to polymorphisms and have no functional impact.
origin :
37
pi :
alt_names
vesicle-associated membrane protein 2
SYB2
Synaptobrevin 2
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5KpnI
promoterCMV
sequencing_primer_3SV40-pA-R
sequencing_primer_5EGFP-C; CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameVAMP 2
shRNA_sequence
size
species
tags
locationN terminal on backbone
tagEGFP
plasmid copy :
Rat Syb2 cDNA from Richard H. Scheller (Stanford University, Stanford, California)
resistance markers :
1431
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA