Zebrafish-gRNA-0007 | Addgene 42247 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

Zebrafish-gRNA-0007

catalog :

42247

citations: 1

Reference
Hwang W, Fu Y, Reyon D, Maeder M, Tsai S, Sander J, et al. Efficient genome editing in zebrafish using a CRISPR-Cas system. Nat Biotechnol. 2013;31:227-9 pubmed publisher

product information

Catalog Number :

42247

Product Name :

Zebrafish-gRNA-0007

article :

doi	10.1038/nbt.2501
id	6371
pubmed_id	23360964

bacterial resistance :

Kanamycin

cloning :

backbone

DR274

backbone_mutation

backbone_origin

Joung lab (Addgene plasmid# 42250)

backbone_size

2147

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

CRISPR

zebrafish expression

growth notes :

TTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGCTTTTAAA For more information on Joung Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/jounglab/
TAATACGACTCACTATAGGATGCGCGTAAGGAGCGCGGT
TTTAGAGCTAGAAATAGCAAG
Target site: GGATGCGCGTAAGGAGCGCG Guide RNA sequence:

origin :

pi :

alt_names

DR317

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter	T7
sequencing_primer_3
sequencing_primer_5	M13 forward
site_3_destroyed
site_5_destroyed

entrez_gene

aliases
gene	th
id	30384

genbank_ids

mutation

name

gRNA-th1

shRNA_sequence

size

species

7955

Danio rerio