This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Zebrafish-gRNA-0005
catalog :
42245
citations: 1
Reference
Hwang W, Fu Y, Reyon D, Maeder M, Tsai S, Sander J, et al. Efficient genome editing in zebrafish using a CRISPR-Cas system. Nat Biotechnol. 2013;31:227-9 pubmed publisher
product information
Catalog Number :
42245
Product Name :
Zebrafish-gRNA-0005
article :
doi10.1038/nbt.2501
id6371
pubmed_id23360964
bacterial resistance :
Kanamycin
cloning :
backboneDR274
backbone_mutation
backbone_originJoung lab (Addgene plasmid# 42250)
backbone_size2147
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
CRISPR
zebrafish expression
growth notes :
TTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGCTTTTAAA For more information on Joung Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/jounglab/
TAATACGACTCACTATAGGGACCTGACCGGCCGCAGGGT
TTTAGAGCTAGAAATAGCAAG
Target site: GGGACCTGACCGGCCGCAGG Guide RNA sequence:
origin :
37
pi :
alt_names
DR338
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterT7
sequencing_primer_3
sequencing_primer_5M13 forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGSK-3[b], GSK3, fk80d11, wu:fb68h05, wu:fk80d11
genegsk3b
id30654
genbank_ids
mutation
namegRNA-gsk3b
shRNA_sequence
size20
species
7955
Danio rerio
tags
resistance markers :
236
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA