This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro
catalog :
42229
citations: 34
Reference
Mavrommatis L, Zaben A, Kindler U, Kienitz M, Dietz J, Jeong H, et al. CRISPR/Cas9 Genome Editing in LGMD2A/R1 Patient-Derived Induced Pluripotent Stem and Skeletal Muscle Progenitor Cells. Stem Cells Int. 2023;2023:9246825 pubmed publisher
Ma M, Dang Y, Chang B, Wang F, Xu J, Chen L, et al. TAK1 is an essential kinase for STING trafficking. Mol Cell. 2023;83:3885-3903.e5 pubmed publisher
Wang F, Zhao M, Chang B, Zhou Y, Wu X, Ma M, et al. Cytoplasmic PARP1 links the genome instability to the inhibition of antiviral immunity through PARylating cGAS. Mol Cell. 2022;82:2032-2049.e7 pubmed publisher
Cui Z, Tian R, Huang Z, Jin Z, Li L, Liu J, et al. FrCas9 is a CRISPR/Cas9 system with high editing efficiency and fidelity. Nat Commun. 2022;13:1425 pubmed publisher
Cui Z, Liu H, Zhang H, Huang Z, Tian R, Li L, et al. The comparison of ZFNs, TALENs, and SpCas9 by GUIDE-seq in HPV-targeted gene therapy. Mol Ther Nucleic Acids. 2021;26:1466-1478 pubmed publisher
Wang G, Li C, He S, Liu Z. Mosaic CRISPR-stop enables rapid phenotyping of nonsense mutations in essential genes. Development. 2021;: pubmed publisher
Chen Y, Jiang H, Wang T, He D, Tian R, Cui Z, et al. In vitro and in vivo growth inhibition of human cervical cancer cells via human papillomavirus E6/E7 mRNAs' cleavage by CRISPR/Cas13a system. Antiviral Res. 2020;178:104794 pubmed publisher
Lustyk D, Kinsky S, Ullrich K, Yancoskie M, Kasikova L, Gergelits V, et al. Genomic Structure of Hstx2 Modifier of Prdm9-Dependent Hybrid Male Sterility in Mice. Genetics. 2019;213:1047-1063 pubmed publisher
Elling R, Robinson E, Shapleigh B, Liapis S, Covarrubias S, Katzman S, et al. Genetic Models Reveal cis and trans Immune-Regulatory Activities for lincRNA-Cox2. Cell Rep. 2018;25:1511-1524.e6 pubmed publisher
Perry R, Hezroni H, Goldrich M, Ulitsky I. Regulation of Neuroregeneration by Long Noncoding RNAs. Mol Cell. 2018;72:553-567.e5 pubmed publisher
Ji H, Lu P, Liu B, Qu X, Wang Y, Jiang Z, et al. Zinc-Finger Nucleases Induced by HIV-1 Tat Excise HIV-1 from the Host Genome in Infected and Latently Infected Cells. Mol Ther Nucleic Acids. 2018;12:67-74 pubmed publisher
Xu J, Li W, Hossen M, Jia Y, Li L, Huang Z. Optimized Plasmid Construction Strategy for Cas9. Cell Physiol Biochem. 2018;48:131-137 pubmed publisher
Stein C, Jadiya P, Zhang X, McLendon J, Abouassaly G, Witmer N, et al. Mitoregulin: A lncRNA-Encoded Microprotein that Supports Mitochondrial Supercomplexes and Respiratory Efficiency. Cell Rep. 2018;23:3710-3720.e8 pubmed publisher
Tsuchiya M, Hara Y, Okuda M, Itoh K, Nishioka R, Shiomi A, et al. Cell surface flip-flop of phosphatidylserine is critical for PIEZO1-mediated myotube formation. Nat Commun. 2018;9:2049 pubmed publisher
Yao X, Zhang M, Wang X, Ying W, Hu X, Dai P, et al. Tild-CRISPR Allows for Efficient and Precise Gene Knockin in Mouse and Human Cells. Dev Cell. 2018;45:526-536.e5 pubmed publisher
Yao X, Wang X, Liu J, Shi L, Huang P, Yang H. CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy. J Vis Exp. 2018;: pubmed publisher
Xu J, Lian W, Jia Y, Li L, Huang Z. Optimized guide RNA structure for genome editing via Cas9. Oncotarget. 2017;8:94166-94171 pubmed publisher
Tan Z, Teo Z, Tan C, Choo C, Loo W, Song Y, et al. ANGPTL4 T266M variant is associated with reduced cancer invasiveness. Biochim Biophys Acta Mol Cell Res. 2017;1864:1525-1536 pubmed publisher
Utani K, Fu H, Jang S, Marks A, Smith O, Zhang Y, et al. Phosphorylated SIRT1 associates with replication origins to prevent excess replication initiation and preserve genomic stability. Nucleic Acids Res. 2017;45:7807-7824 pubmed publisher
Colin York H, Eggeling C, Fritzsche M. Dissection of mechanical force in living cells by super-resolved traction force microscopy. Nat Protoc. 2017;12:783-796 pubmed publisher
Hallmann A, Arauzo Bravo M, Mavrommatis L, Ehrlich M, Röpke A, Brockhaus J, et al. Astrocyte pathology in a human neural stem cell model of frontotemporal dementia caused by mutant TAU protein. Sci Rep. 2017;7:42991 pubmed publisher
Dong F, Xie K, Chen Y, Yang Y, Mao Y. Polycistronic tRNA and CRISPR guide-RNA enables highly efficient multiplexed genome engineering in human cells. Biochem Biophys Res Commun. 2017;482:889-895 pubmed publisher
Kaminski R, Chen Y, Salkind J, Bella R, Young W, Ferrante P, et al. Negative Feedback Regulation of HIV-1 by Gene Editing Strategy. Sci Rep. 2016;6:31527 pubmed publisher
Dai L, Chen K, Youngren B, Kulina J, Yang A, Guo Z, et al. Cytoplasmic Drosha activity generated by alternative splicing. Nucleic Acids Res. 2016;44:10454-10466 pubmed
van Diemen F, Kruse E, Hooykaas M, Bruggeling C, Schürch A, van Ham P, et al. CRISPR/Cas9-Mediated Genome Editing of Herpesviruses Limits Productive and Latent Infections. PLoS Pathog. 2016;12:e1005701 pubmed publisher
Wang S, Su J, Zhang F, Zhuang X. An RNA-aptamer-based two-color CRISPR labeling system. Sci Rep. 2016;6:26857 pubmed publisher
Rennoll S, Eshelman M, Raup Konsavage W, Kawasawa Y, Yochum G. The MYC 3' Wnt-Responsive Element Drives Oncogenic MYC Expression in Human Colorectal Cancer Cells. Cancers (Basel). 2016;8: pubmed publisher
Maure J, Moser S, Jaffray E, F Alpi A, Hay R. Loss of ubiquitin E2 Ube2w rescues hypersensitivity of Rnf4 mutant cells to DNA damage. Sci Rep. 2016;6:26178 pubmed publisher
Roehm P, Shekarabi M, Wollebo H, Bellizzi A, He L, Salkind J, et al. Inhibition of HSV-1 Replication by Gene Editing Strategy. Sci Rep. 2016;6:23146 pubmed publisher
Harms D, Quadros R, Seruggia D, Ohtsuka M, Takahashi G, Montoliu L, et al. Mouse Genome Editing Using the CRISPR/Cas System. Curr Protoc Hum Genet. 2014;83:15.7.1-27 pubmed publisher
Gonzalez F, Zhu Z, Shi Z, Lelli K, Verma N, Li Q, et al. An iCRISPR platform for rapid, multiplexable, and inducible genome editing in human pluripotent stem cells. Cell Stem Cell. 2014;15:215-226 pubmed publisher
Zhao Y, Dai Z, Liang Y, Yin M, Ma K, He M, et al. Sequence-specific inhibition of microRNA via CRISPR/CRISPRi system. Sci Rep. 2014;4:3943 pubmed publisher
Upadhyay S, Kumar J, Alok A, Tuli R. RNA-guided genome editing for target gene mutations in wheat. G3 (Bethesda). 2013;3:2233-8 pubmed publisher
Cong L, Ran F, Cox D, Lin S, Barretto R, Habib N, et al. Multiplex genome engineering using CRISPR/Cas systems. Science. 2013;339:819-23 pubmed publisher
product information
Catalog Number :
42229
Product Name :
pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro
article :
doi10.1126/science.1231143
id6225
pubmed_id23287718
bacterial resistance :
Ampicillin
cloning :
backbonepUC ori vector
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
CRISPR
growth notes :
Note, Addgene's quality control sequencing has found a few discrepancies with the depositor's full sequence. The depositing lab is aware of these discrepancies, and they are not thought to affect plasmid function. For more information on Zhang Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/zhang/
growth strain :
This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA.
origin :
37
pi :
alt_names
SpCas9
hSpCas9
cloning
entrez_gene
genbank_ids
mutation
namehumanized S. pyogenes Cas9
shRNA_sequence
size4272
species
tags
locationN terminal on insert
tag3xFLAG
resistance markers :
898
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA