pcDNA3 c-SRC (250-536) | Addgene 42208 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA3 c-SRC (250-536)

catalog :

42208

citations: 2

Reference
Fewou S, Röckle I, Hildebrandt H, Eckhardt M. Transgenic overexpression of polysialyltransferase ST8SiaIV under the control of a neuron-specific promoter does not affect brain development but impairs exploratory behavior. Glycobiology. 2019;29:657-668 pubmed publisher
Miller W, Maudsley S, Ahn S, Khan K, Luttrell L, Lefkowitz R. beta-arrestin1 interacts with the catalytic domain of the tyrosine kinase c-SRC. Role of beta-arrestin1-dependent targeting of c-SRC in receptor endocytosis. J Biol Chem. 2000;275:11312-9 pubmed

product information

Catalog Number :

42208

Product Name :

pcDNA3 c-SRC (250-536)

article :

doi	10.1074/jbc.275.15.11312
id	6207
pubmed_id	10753943

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5446

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

All c-SRC mutants were generated by polymerase chain reaction using c-SRC (WT) (Addgene Plasmid #42202) as a template, and polymerase chain reaction products containing a minimal Kozak sequence were cloned into the HindIII and XhoI sites of pcDNA3. This construct contains amino acids 250-536 of human c-SRC and is untagged. All sequences were confirmed using an automated ABI DNA sequencer (Howard Hughes Nucleic Acid Facility, Duke University).

origin :

pi :

alt_names

SRC

sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog

p60-Src

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	HindIII
promoter	CMV
sequencing_primer_3	BGH-rev
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ASV, SRC1, THC6, c-SRC, p60-Src
gene	SRC
id	6714

genbank_ids

NM_005417.3

AAA60584.1

mutation

contains amino acids 250-536

name

c-SRC (250-536)

shRNA_sequence

size

species

9606

Homo sapiens