pcDNA3 c-SRC (151-536) | Addgene 42207 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA3 c-SRC (151-536)

catalog :

42207

citations: 2

Reference
Iida M, Sahashi K, Kondo N, Nakatsuji H, Tohnai G, Tsutsumi Y, et al. Src inhibition attenuates polyglutamine-mediated neuromuscular degeneration in spinal and bulbar muscular atrophy. Nat Commun. 2019;10:4262 pubmed publisher
Miller W, Maudsley S, Ahn S, Khan K, Luttrell L, Lefkowitz R. beta-arrestin1 interacts with the catalytic domain of the tyrosine kinase c-SRC. Role of beta-arrestin1-dependent targeting of c-SRC in receptor endocytosis. J Biol Chem. 2000;275:11312-9 pubmed

product information

Catalog Number :

42207

Product Name :

pcDNA3 c-SRC (151-536)

article :

doi	10.1074/jbc.275.15.11312
id	6207
pubmed_id	10753943

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5446

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

All c-SRC mutants were generated by polymerase chain reaction using c-SRC (WT) (Addgene Plasmid #42202) as a template, and polymerase chain reaction products containing a minimal Kozak sequence were cloned into the HindIII and XhoI sites of pcDNA3. This construct contains amino acids 151-536 of human c-SRC and is untagged. All sequences were confirmed using an automated ABI DNA sequencer (Howard Hughes Nucleic Acid Facility, Duke University). This plasmid also contains an L176F point mutation which should not affect function.

origin :

pi :

alt_names

SRC

sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog

p60-Src

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	HindIII
promoter	CMV
sequencing_primer_3	BGH-rev
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ASV, SRC1, THC6, c-SRC, p60-Src
gene	SRC
id	6714

genbank_ids

NM_005417.3

AAA60584.1

mutation

contains amino acids 151-536

name

c-SRC (151-536)

shRNA_sequence

size

species

9606

Homo sapiens