This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Fz4-GFP
catalog :
42197
citations: 5
Reference
Wesslowski J, Kozielewicz P, Wang X, Cui H, Schihada H, Kranz D, et al. eGFP-tagged Wnt-3a enables functional analysis of Wnt trafficking and signaling and kinetic assessment of Wnt binding to full-length Frizzled. J Biol Chem. 2020;295:8759-8774 pubmed publisher
Bachg A, Horsthemke M, Skryabin B, Klasen T, Nagelmann N, Faber C, et al. Phenotypic analysis of Myo10 knockout (Myo10tm2/tm2) mice lacking full-length (motorized) but not brain-specific headless myosin X. Sci Rep. 2019;9:597 pubmed publisher
Eubelen M, Bostaille N, Cabochette P, Gauquier A, Tebabi P, Dumitru A, et al. A molecular mechanism for Wnt ligand-specific signaling. Science. 2018;361: pubmed publisher
Skronska Wasek W, Mutze K, Baarsma H, Bracke K, Alsafadi H, Lehmann M, et al. Reduced Frizzled Receptor 4 Expression Prevents WNT/?-Catenin-driven Alveolar Lung Repair in Chronic Obstructive Pulmonary Disease. Am J Respir Crit Care Med. 2017;196:172-185 pubmed publisher
Chen W, ten Berge D, Brown J, Ahn S, Hu L, Miller W, et al. Dishevelled 2 recruits beta-arrestin 2 to mediate Wnt5A-stimulated endocytosis of Frizzled 4. Science. 2003;301:1391-4 pubmed
product information
Catalog Number :
42197
Product Name :
Fz4-GFP
article :
doi10.1126/science.1082808
id6204
pubmed_id12958364
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-N2
backbone_mutation
backbone_originClontech
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Fz4-GFP was generated by fusing EGFP (Clontech) at its C-terminus. Alternate plasmid names: pEGFPN2 mFz4; pEGFP-N2-Frizzled4
origin :
37
pi :
alt_names
Fz4
frizzled homolog 4
Fzd4
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3EGFP-N, SV40pA-R
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesFz4
geneFzd4
id14366
genbank_ids
NM_008055.4
NP_032081.3
mutation
nameFrizzled 4
shRNA_sequence
size
species
10090
Mus musculus
tags
locationC terminal on backbone
tagEGFP
resistance markers :
285
983
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA