pcDNA3 arr1 S412A Flag | Addgene 42195 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA3 arr1 S412A Flag

catalog :

42195

citations: 1

Reference
Luttrell L, Ferguson S, Daaka Y, Miller W, Maudsley S, Della Rocca G, et al. Beta-arrestin-dependent formation of beta2 adrenergic receptor-Src protein kinase complexes. Science. 1999;283:655-61 pubmed

product information

Catalog Number :

42195

Product Name :

pcDNA3 arr1 S412A Flag

article :

doi	10.1126/science.283.5402.655
id	1329
pubmed_id	9924018

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5446

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Flag epitope-tagged truncation mutants of -arrestin 1 were prepared by the polymerase chain reaction (PCR), incorporating an Eco RI site, minimal Kozak sequence (ACC), and initiator methionine codon into the 5' primer, and the Flag epitope sequence, stop codon, and an Xho I site into the 3' primer. PCR products were subcloned into pcDNA3. DNA sequences were confirmed by dideoxynucleotide sequencing.

origin :

pi :

alt_names

-arrestin 1

Beta arrestin 1

arrb1

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	EcoRI
promoter	CMV
sequencing_primer_3	BGH-rev
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	BARRES
gene	Arrb1
id	25387

genbank_ids

NM_012910.2

NP_037042.1

mutation

S412A

name

-arrestin 1 S412A

shRNA_sequence

size

1300

species

10116

Rattus norvegicus