slpr genomic fragment | Addgene 42074 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

slpr genomic fragment

catalog :

42074

citations: 1

Reference
Stronach B, Perrimon N. Activation of the JNK pathway during dorsal closure in Drosophila requires the mixed lineage kinase, slipper. Genes Dev. 2002;16:377-87 pubmed

product information

Catalog Number :

42074

Product Name :

slpr genomic fragment

article :

doi	10.1101/gad.953002
id	6168
pubmed_id	11825878

bacterial resistance :

Ampicillin

cloning :

backbone

pCaSpeR4

backbone_mutation

backbone_origin

backbone_size

7862

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Insect Expression

growth notes :

The slpr genomic rescue construct was generated by isolating a HindIII fragment of 14 kb from the P1 clone DS08402, including the MLK gene, and a neighboring annotated geneCG15339. This fragment was cloned transiently into pBluescript (Stratagene) and then moved into pCaSpeR4 vector for subsequent purification and germ-line transformation.

origin :

pi :

alt_names

slipper

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter	Hsp70
sequencing_primer_3	pCasper-R
sequencing_primer_5	pCasper-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Dmel_CG2272, CG2272, D-MLK, Dmel CG2272, JNKKK, MLK, MLK2, Mlk2, Slpr, dMLK, dMLK2
gene	slpr
id	44111

genbank_ids

mutation

name

slpr genomic fragment

shRNA_sequence

size

species

7227

Drosophila melanogaster