This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBABE puro Brca1 S1423A S1524A HA
catalog :
41968
citations: 2
Reference
Li Q, Hao Q, Cao W, Li J, Wu K, Elshimali Y, et al. PP2Cδ inhibits p300-mediated p53 acetylation via ATM/BRCA1 pathway to impede DNA damage response in breast cancer. Sci Adv. 2019;5:eaaw8417 pubmed publisher
Cortez D, Wang Y, Qin J, Elledge S. Requirement of ATM-dependent phosphorylation of brca1 in the DNA damage response to double-strand breaks. Science. 1999;286:1162-6 pubmed
product information
Catalog Number :
41968
Product Name :
pBABE puro Brca1 S1423A S1524A HA
article :
doi10.1126/science.286.5442.1162
id1421
pubmed_id10550055
bacterial resistance :
Ampicillin
cloning :
backbonepBABE-puro
backbone_mutation
backbone_origin
backbone_size5200
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
The BRCA1 insert in this plasmid contains the following common polymorphisms when compared to GenBank reference sequence NP_009225.1: P871L, E1038G, K1182R, and S1613G. These SNPs were likely present in the original cDNA isolate.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3Sal1
cloning_site_5Sal1
promoter
sequencing_primer_3pBABE 3'
sequencing_primer_5pBABE 5'
site_3_destroyed1
site_5_destroyed
entrez_gene
aliasesBRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53
geneBRCA1
id672
genbank_ids
mutationS1423A, S1524A
nameBRCA1
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHA
plasmid copy :
D. Livingston, DFCI
resistance markers :
133
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA