This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAG306-GPD-VMA1 chr I
catalog :
41896
citations: 1
Reference
Hughes A, Gottschling D. An early age increase in vacuolar pH limits mitochondrial function and lifespan in yeast. Nature. 2012;492:261-5 pubmed publisher
product information
Catalog Number :
41896
Product Name :
pAG306-GPD-VMA1 chr I
article :
doi10.1038/nature11654
id6141
pubmed_id23172144
bacterial resistance :
Ampicillin
cloning :
backbonepAG306-GPD-ccdB chr I
backbone_mutationInserted VMA1 using LR clonase
backbone_originDan Gottschling (Addgene plasmid# 41894)
backbone_size7882
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth strain :
Yeast expression of VMA1.
origin :
37
pi :
alt_names
TFP1
Vma1p
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterGPD
sequencing_primer_3pBluescriptKS_primer
sequencing_primer_5pBluescriptSK_primer
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYDL185W, CLS8, TFP1
geneVMA1
id851342
genbank_ids
NM_001180245
mutationContains silent mutation at 342A-G and L87S mutation in VMA1; 2144A-G mutation in SceI intein
nameVMA1
shRNA_sequence
size
species
4932
Saccharomyces cerevisiae
tags
plasmid copy :
VMA1 from donor Gateway plasmid pDONR221-VMA1, Harvard Institute of Proteomics (HIP) accession ScCD00023712
resistance markers :
1416
tags :
Unknown
terms :
URA3
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA