This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
psiCheck-ApoECDS
catalog :
41848
citations: 1
Reference
Pencheva N, Tran H, Buss C, Huh D, Drobnjak M, Busam K, et al. Convergent multi-miRNA targeting of ApoE drives LRP1/LRP8-dependent melanoma metastasis and angiogenesis. Cell. 2012;151:1068-82 pubmed publisher
product information
Catalog Number :
41848
Product Name :
psiCheck-ApoECDS
article :
doi10.1016/j.cell.2012.10.028
id6129
pubmed_id23142051
bacterial resistance :
Ampicillin
cloning :
backbonePsiCheck2
backbone_mutationThe CDS of ApoE is chimerically fused to the C terminus of the renilla luciferase reporter in the psiCheck2 vector by removing the luciferase stop codon and inserting a 6 amino acid-long linker connecting the CDS of luciferase to the CDS of ApoE.
backbone_originPromega
backbone_size6273
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Luciferase
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5XhoI
promoter
sequencing_primer_3AAGACTCATTTAGATCCTCACAC
sequencing_primer_5GTACATCAAGAGCTTCGTGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAD2, APO-E, ApoE4, LDLCQ5, LPG
geneAPOE
id348
genbank_ids
NM_000041
mutation
nameApoE CDS
shRNA_sequence
size954
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagrenilla luciferase
resistance markers :
1411
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA