This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
hCas9_D10A
catalog :
41816
citations: 35
Reference
Nie J, Ueda Y, Solivais A, Hashino E. CHD7 regulates otic lineage specification and hair cell differentiation in human inner ear organoids. Nat Commun. 2022;13:7053 pubmed publisher
Habu T, Ishikawa H, Kim J. Gulo gene locus, a new gene editing locus for mammalian cells. Biotechnol J. 2022;:e2100493 pubmed publisher
Pallarès Masmitjà M, Ivančić D, Mir Pedrol J, Jaraba Wallace J, Tagliani T, Oliva B, et al. Find and cut-and-transfer (FiCAT) mammalian genome engineering. Nat Commun. 2021;12:7071 pubmed publisher
Laigle V, Dingli F, Amhaz S, Perron T, Chouchène M, Colasse S, et al. Quantitative Ubiquitylome Analysis Reveals the Specificity of RNF111/Arkadia E3 Ubiquitin Ligase for its Degradative Substrates SKI and SKIL/SnoN in TGF-β Signaling Pathway. Mol Cell Proteomics. 2021;20:100173 pubmed publisher
Chen Z, Wang J, Kang Y, Yang Q, Gu X, Zhi D, et al. PINK1 gene mutation by pair truncated sgRNA/Cas9-D10A in cynomolgus monkeys. Zool Res. 2021;42:469-477 pubmed publisher
Baldassarre M, Solano Collado V, Balci A, Colamarino R, Dambuza I, Reid D, et al. The Rab32/BLOC-3-dependent pathway mediates host defense against different pathogens in human macrophages. Sci Adv. 2021;7: pubmed publisher
Bondarieva A, Raveendran K, Telychko V, Rao H, Ravindranathan R, Zorzompokou C, et al. Proline-rich protein PRR19 functions with cyclin-like CNTD1 to promote meiotic crossing over in mouse. Nat Commun. 2020;11:3101 pubmed publisher
Woo Y, Kim S, Suh B, Kwak Y, Jung H, Nhung T, et al. Sequential phosphorylation of NDEL1 by the DYRK2-GSK3β complex is critical for neuronal morphogenesis. elife. 2019;8: pubmed publisher
Winter J, Luu A, Gapinske M, Manandhar S, Shirguppe S, Woods W, et al. Targeted exon skipping with AAV-mediated split adenine base editors. Cell Discov. 2019;5:41 pubmed publisher
Prorok P, Artufel M, Aze A, Coulombe P, Peiffer I, Lacroix L, et al. Involvement of G-quadruplex regions in mammalian replication origin activity. Nat Commun. 2019;10:3274 pubmed publisher
Sheng C, Mendler I, Rieke S, Snyder P, Jentsch M, Friedrich D, et al. PCNA-Mediated Degradation of p21 Coordinates the DNA Damage Response and Cell Cycle Regulation in Individual Cells. Cell Rep. 2019;27:48-58.e7 pubmed publisher
Pflueger C, Tan D, Swain T, Nguyen T, Pflueger J, Nefzger C, et al. A modular dCas9-SunTag DNMT3A epigenome editing system overcomes pervasive off-target activity of direct fusion dCas9-DNMT3A constructs. Genome Res. 2018;28:1193-1206 pubmed publisher
Li P, Marino M, Zou J, Argaw T, Morreale M, Iaffaldano B, et al. Efficiency and Specificity of Targeted Integration Mediated by the Adeno-Associated Virus Serotype 2 Rep 78 Protein. Hum Gene Ther Methods. 2018;29:135-145 pubmed publisher
Ribeiro de Almeida C, Dhir S, Dhir A, Moghaddam A, Sattentau Q, Meinhart A, et al. RNA Helicase DDX1 Converts RNA G-Quadruplex Structures into R-Loops to Promote IgH Class Switch Recombination. Mol Cell. 2018;70:650-662.e8 pubmed publisher
Böiers C, Richardson S, Laycock E, Zriwil A, Turati V, Brown J, et al. A Human IPS Model Implicates Embryonic B-Myeloid Fate Restriction as Developmental Susceptibility to B Acute Lymphoblastic Leukemia-Associated ETV6-RUNX1. Dev Cell. 2018;44:362-377.e7 pubmed publisher
Ade C, Derbes R, Wagstaff B, Linker S, White T, Deharo D, et al. Evaluating different DNA binding domains to modulate L1 ORF2p-driven site-specific retrotransposition events in human cells. Gene. 2018;642:188-198 pubmed publisher
Chen X, Janssen J, Liu J, Maggio I, t Jong A, Mikkers H, et al. In trans paired nicking triggers seamless genome editing without double-stranded DNA cutting. Nat Commun. 2017;8:657 pubmed publisher
Yue J, Gou X, Li Y, Wicksteed B, Wu X. Engineered Epidermal Progenitor Cells Can Correct Diet-Induced Obesity and Diabetes. Cell Stem Cell. 2017;21:256-263.e4 pubmed publisher
Koehler K, Nie J, Longworth Mills E, Liu X, Lee J, Holt J, et al. Generation of inner ear organoids containing functional hair cells from human pluripotent stem cells. Nat Biotechnol. 2017;35:583-589 pubmed publisher
Iefremova V, Manikakis G, Krefft O, Jabali A, Weynans K, Wilkens R, et al. An Organoid-Based Model of Cortical Development Identifies Non-Cell-Autonomous Defects in Wnt Signaling Contributing to Miller-Dieker Syndrome. Cell Rep. 2017;19:50-59 pubmed publisher
Kan Y, Ruis B, Takasugi T, Hendrickson E. Mechanisms of precise genome editing using oligonucleotide donors. Genome Res. 2017;27:1099-1111 pubmed publisher
Wen Y, Liao G, PRITCHARD T, Zhao T, Connelly J, Pruett Miller S, et al. A stable but reversible integrated surrogate reporter for assaying CRISPR/Cas9-stimulated homology-directed repair. J Biol Chem. 2017;292:6148-6162 pubmed publisher
Park K, Powell A, Sandmaier S, Kim C, Mileham A, Donovan D, et al. Targeted gene knock-in by CRISPR/Cas ribonucleoproteins in porcine zygotes. Sci Rep. 2017;7:42458 pubmed publisher
Osborn M, Lonetree C, Webber B, Patel D, Dunmire S, McElroy A, et al. CRISPR/Cas9 Targeted Gene Editing and Cellular Engineering in Fanconi Anemia. Stem Cells Dev. 2016;25:1591-1603 pubmed
Vriend L, Prakash R, Chen C, Vanoli F, Cavallo F, Zhang Y, et al. Distinct genetic control of homologous recombination repair of Cas9-induced double-strand breaks, nicks and paired nicks. Nucleic Acids Res. 2016;44:5204-17 pubmed publisher
Terao M, Tamano M, Hara S, Kato T, Kinoshita M, Takada S. Utilization of the CRISPR/Cas9 system for the efficient production of mutant mice using crRNA/tracrRNA with Cas9 nickase and FokI-dCas9. Exp Anim. 2016;65:275-83 pubmed publisher
He X, Tan C, Wang F, Wang Y, Zhou R, Cui D, et al. Knock-in of large reporter genes in human cells via CRISPR/Cas9-induced homology-dependent and independent DNA repair. Nucleic Acids Res. 2016;44:e85 pubmed publisher
Hara S, Tamano M, Yamashita S, Kato T, Saito T, Sakuma T, et al. Generation of mutant mice via the CRISPR/Cas9 system using FokI-dCas9. Sci Rep. 2015;5:11221 pubmed publisher
O Geen H, Henry I, Bhakta M, Meckler J, Segal D. A genome-wide analysis of Cas9 binding specificity using ChIP-seq and targeted sequence capture. Nucleic Acids Res. 2015;43:3389-404 pubmed publisher
Lin J, Chen H, Luo L, Lai Y, Xie W, Kee K. Creating a monomeric endonuclease TALE-I-SceI with high specificity and low genotoxicity in human cells. Nucleic Acids Res. 2015;43:1112-22 pubmed publisher
Inui M, Miyado M, Igarashi M, Tamano M, Kubo A, Yamashita S, et al. Rapid generation of mouse models with defined point mutations by the CRISPR/Cas9 system. Sci Rep. 2014;4:5396 pubmed publisher
Maggio I, Holkers M, Liu J, Janssen J, Chen X, Gonçalves M. Adenoviral vector delivery of RNA-guided CRISPR/Cas9 nuclease complexes induces targeted mutagenesis in a diverse array of human cells. Sci Rep. 2014;4:5105 pubmed publisher
Hu J, Lei Y, Wong W, Liu S, Lee K, He X, et al. Direct activation of human and mouse Oct4 genes using engineered TALE and Cas9 transcription factors. Nucleic Acids Res. 2014;42:4375-90 pubmed publisher
Fujita T, Fujii H. Efficient isolation of specific genomic regions and identification of associated proteins by engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR. Biochem Biophys Res Commun. 2013;439:132-6 pubmed publisher
Mali P, Yang L, Esvelt K, Aach J, Güell M, Dicarlo J, et al. RNA-guided human genome engineering via Cas9. Science. 2013;339:823-6 pubmed publisher
product information
Catalog Number :
41816
Product Name :
hCas9_D10A
article :
doi10.1126/science.1232033
id6213
pubmed_id23287722
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.3-TOPO
backbone_mutation
backbone_originInvitrogen
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
CRISPR
growth notes :
For additional information: http://arep.med.harvard.edu/human_crispr/ For more information on Church Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/church/
growth strain :
Expresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineering
origin :
37
pi :
alt_names
cloning
clone_methodTOPO Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3
sequencing_primer_5CMV_F
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutationhuman codon-optimized, D10A nickase
nameCas9_D10A
shRNA_sequence
size4140
species
tags
resistance markers :
765
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA