This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
hCas9
catalog :
41815
citations: 373
Reference
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Yuan F, Xu Y, You K, Zhang J, Yang F, Li Y. Calcitriol alleviates ethanol-induced hepatotoxicity via AMPK/mTOR-mediated autophagy. Arch Biochem Biophys. 2021;697:108694 pubmed publisher
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Chung C, Allen A, Atkins A, Sullivan N, Homan G, Costello R, et al. Safe CRISPR-Cas9 Inhibition of HIV-1 with High Specificity and Broad-Spectrum Activity by Targeting LTR NF-κB Binding Sites. Mol Ther Nucleic Acids. 2020;21:965-982 pubmed publisher
Fan J, You L, Wang W, Huang W, Chu C, Chi Y, et al. Lamin A-mediated nuclear lamina integrity is required for proper ciliogenesis. EMBO Rep. 2020;21:e49680 pubmed publisher
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Huang X, Chen Z, Liu Y. RNAi-mediated control of CRISPR functions. Theranostics. 2020;10:6661-6673 pubmed publisher
Hamaker N, Lee K. A Site-Specific Integration Reporter System That Enables Rapid Evaluation of CRISPR/Cas9-Mediated Genome Editing Strategies in CHO Cells. Biotechnol J. 2020;:e2000057 pubmed publisher
Rose J, Popp N, Richardson C, Stephany J, Mathieu J, Wei C, et al. Suppression of unwanted CRISPR-Cas9 editing by co-administration of catalytically inactivating truncated guide RNAs. Nat Commun. 2020;11:2697 pubmed publisher
Alomairi J, Molitor A, Sadouni N, Hussain S, Torres M, Saadi W, et al. Integration of high-throughput reporter assays identify a critical enhancer of the Ikzf1 gene. PLoS ONE. 2020;15:e0233191 pubmed publisher
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Xi H, Langerman J, Sabri S, Chien P, Young C, Younesi S, et al. A Human Skeletal Muscle Atlas Identifies the Trajectories of Stem and Progenitor Cells across Development and from Human Pluripotent Stem Cells. Cell Stem Cell. 2020;27:158-176.e10 pubmed publisher
Rui Y, Varanasi M, Mendes S, Yamagata H, Wilson D, Green J. Poly(Beta-Amino Ester) Nanoparticles Enable Nonviral Delivery of CRISPR-Cas9 Plasmids for Gene Knockout and Gene Deletion. Mol Ther Nucleic Acids. 2020;20:661-672 pubmed publisher
Smith C, Castanon O, Said K, Volf V, Khoshakhlagh P, Hornick A, et al. Enabling large-scale genome editing at repetitive elements by reducing DNA nicking. Nucleic Acids Res. 2020;48:5183-5195 pubmed publisher
Puteri M, Watanabe Y, Wardhani B, Amalia R, Abdelaziz M, Kato M. PMEPA1/TMEPAI isoforms function via its PY and Smad-interaction motifs for tumorigenic activities of breast cancer cells. Genes Cells. 2020;25:375-390 pubmed publisher
Wardhani B, Puteri M, Watanabe Y, Louisa M, Setiabudy R, Kato M. TGF-β-Induced TMEPAI Attenuates the Response of Triple-Negative Breast Cancer Cells to Doxorubicin and Paclitaxel. J Exp Pharmacol. 2020;12:17-26 pubmed publisher
Ordureau A, Paulo J, Zhang J, An H, Swatek K, Cannon J, et al. Global Landscape and Dynamics of Parkin and USP30-Dependent Ubiquitylomes in iNeurons during Mitophagic Signaling. Mol Cell. 2020;77:1124-1142.e10 pubmed publisher
Forbester J, Clement M, Wellington D, Yeung A, Dimonte S, Marsden M, et al. IRF5 Promotes Influenza Virus-Induced Inflammatory Responses in Human Induced Pluripotent Stem Cell-Derived Myeloid Cells and Murine Models. J Virol. 2020;94: pubmed publisher
Nozawa T, Sano S, Minowa Nozawa A, Toh H, Nakajima S, Murase K, et al. TBC1D9 regulates TBK1 activation through Ca2+ signaling in selective autophagy. Nat Commun. 2020;11:770 pubmed publisher
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Manirujjaman M, Ozaki I, Murata Y, Guo J, Xia J, Nishioka K, et al. Degradation of the Tumor Suppressor PDCD4 Is Impaired by the Suppression of p62/SQSTM1 and Autophagy. Cells. 2020;9: pubmed publisher
Hara S, Terao M, Muramatsu A, Takada S. Efficient production and transmission of CRISPR/Cas9-mediated mutant alleles at the IG-DMR via generation of mosaic mice using a modified 2CC method. Sci Rep. 2019;9:20202 pubmed publisher
Shinoda K, Maman Y, Canela A, Schatz D, Livak F, Nussenzweig A. Intra-Vκ Cluster Recombination Shapes the Ig Kappa Locus Repertoire. Cell Rep. 2019;29:4471-4481.e6 pubmed publisher
Novellasdemunt L, Kucharska A, Jamieson C, Prange Barczynska M, Baulies A, Antas P, et al. NEDD4 and NEDD4L regulate Wnt signalling and intestinal stem cell priming by degrading LGR5 receptor. EMBO J. 2019;:e102771 pubmed publisher
Toh H, Lin C, Nakajima S, Aikawa C, Nozawa T, Nakagawa I. Group A Streptococcus NAD-Glycohydrolase Inhibits Caveolin 1-Mediated Internalization Into Human Epithelial Cells. Front Cell Infect Microbiol. 2019;9:398 pubmed publisher
Oh hashi K, Kohno H, Kandeel M, Hirata Y. Characterization of IRE1α in Neuro2a cells by pharmacological and CRISPR/Cas9 approaches. Mol Cell Biochem. 2020;465:53-64 pubmed publisher
Isobe M, Toya H, Mito M, Chiba T, Asahara H, Hirose T, et al. Forced isoform switching of Neat1_1 to Neat1_2 leads to the loss of Neat1_1 and the hyperformation of paraspeckles but does not affect the development and growth of mice. RNA. 2020;26:251-264 pubmed publisher
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Saito K, Iioka H, Maruyama S, Sumardika I, Sakaguchi M, Kondo E. PODXL1 promotes metastasis of the pancreatic ductal adenocarcinoma by activating the C5aR/C5a axis from the tumor microenvironment. Neoplasia. 2019;21:1121-1132 pubmed publisher
Sullivan N, Dampier W, Chung C, Allen A, Atkins A, Pirrone V, et al. Novel gRNA design pipeline to develop broad-spectrum CRISPR/Cas9 gRNAs for safe targeting of the HIV-1 quasispecies in patients. Sci Rep. 2019;9:17088 pubmed publisher
Chung J, Wu X, Lambert T, Lai Z, Walther T, Farese R. LDAF1 and Seipin Form a Lipid Droplet Assembly Complex. Dev Cell. 2019;51:551-563.e7 pubmed publisher
Morishige S, Mizuno S, Ozawa H, Nakamura T, Mazahery A, Nomura K, et al. CRISPR/Cas9-mediated gene correction in hemophilia B patient-derived iPSCs. Int J Hematol. 2019;: pubmed publisher
Lin Z, Li Y, Jin Y, Kim N. A Maternal Transcription Factor, Junction Mediating and Regulatory Protein is Required for Preimplantation Development in the Mouse. Dev Reprod. 2019;23:285-295 pubmed publisher
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Catalog Number :
41815
Product Name :
hCas9
article :
doi10.1126/science.1232033
id6213
pubmed_id23287722
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.3-TOPO
backbone_mutation
backbone_originInvitrogen
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
CRISPR
growth notes :
For additional information: http://arep.med.harvard.edu/human_crispr/ For more information on Church Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/church/
growth strain :
Expresses human codon optimized Cas9 nuclease for genome engineering
origin :
37
pi :
alt_names
cloning
clone_methodTOPO Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3
sequencing_primer_5CMV_F
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutationhuman codon-optimized
nameCas9
shRNA_sequence
size4140
species
tags
resistance markers :
765
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA