This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
H9 pMalLP
catalog :
41811
citations: 1
Reference
Levin A, Bates D, Ring A, Krieg C, Lin J, Su L, et al. Exploiting a natural conformational switch to engineer an interleukin-2 'superkine'. Nature. 2012;484:529-33 pubmed publisher
product information
Catalog Number :
41811
Product Name :
H9 pMalLP
article :
doi10.1038/nature10975
id6117
pubmed_id22446627
bacterial resistance :
Ampicillin
cloning :
backbonepMalLP
backbone_mutationThe vector is a variant of pMalp2x, with a modified MCS and an N-terminal 3C protease cleavage site.
backbone_origin
backbone_size6700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
This is an e. coli periplasmic expression vector. H9 has the following mutations compared to wildtype IL-2: L80F, R81F, L85V, I86V, I92F
origin :
37
pi :
alt_names
H9
Super-2
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5EcoRI
promoterpTac
sequencing_primer_3M13-65
sequencing_primer_5Mal-E
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesIL-2, TCGF, lymphokine
geneIL2
id3558
genbank_ids
mutation
nameH9 Super-2
shRNA_sequence
size429
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagMaltose Binding Protein
locationN terminal on backbone
tag3C protease site
locationC terminal on insert
tag6x His
resistance markers :
1408
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA