pEF-Bos TRIF Flag | Addgene 41550 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pEF-Bos TRIF Flag

catalog :

41550

citations: 4

Reference
Murugan S, Nandi B, Mazumdar V, Joshi K, Nandini P, Namani S, et al. Outer membrane protein 25 of Brucella suppresses TLR-mediated expression of proinflammatory cytokines through degradation of TLRs and adaptor proteins. J Biol Chem. 2023;299:105309 pubmed publisher
Deng M, Tam J, Wang L, Liang K, Li S, Zhang L, et al. TRAF3IP3 negatively regulates cytosolic RNA induced anti-viral signaling by promoting TBK1 K48 ubiquitination. Nat Commun. 2020;11:2193 pubmed publisher
Kim J, Kim Y, Stinski M, Ahn J, Song Y. Human Cytomegalovirus IE2 86 kDa Protein Induces STING Degradation and Inhibits cGAMP-Mediated IFN-Î² Induction. Front Microbiol. 2017;8:1854 pubmed publisher
Fitzgerald K, McWhirter S, Faia K, Rowe D, Latz E, Golenbock D, et al. IKKepsilon and TBK1 are essential components of the IRF3 signaling pathway. Nat Immunol. 2003;4:491-6 pubmed

product information

Catalog Number :

41550

Product Name :

pEF-Bos TRIF Flag

article :

doi	10.1038/ni921
id	3630
pubmed_id	12692549

bacterial resistance :

Ampicillin

cloning :

backbone

pEF-Bos

backbone_mutation

backbone_origin

Mizushima and Nagata (Osaka Bioscience Institute, Japan, 1990) (PMID: 1698283)

backbone_size

5685

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

The depositing lab generated pEF-Bos TRIF Flag from a human peripheral blood mononuclear cell (PBMC) complementary DNA library by PCR amplification and cloning. Due to the facts that the fusion site into the FLAG/His is a BamHI site and that TRIF has both an internal BamHI and a BglII site, the depositing lab used an Esp3I site to create an overhang compatible with the 3' BamHI site. XhoI was used for cloning at the 5' end. NotI can be used at the 3' end to excise the tagged CDS. The construct also contains a HIS tag. Esp3I has the following cutting pattern: CGTCTCNNNNN GCAGAGN In the case of TRIF, the fusion results in a junction that resembles a BglII/BamHI fusion, and is therefore unable to be recut.

origin :

pi :

alt_names

TIR domain containing adapter-inducing IFN

TICAM-1

cloning

clone_method	Restriction Enzyme
cloning_site_3	Esp3/BamHI
cloning_site_5	XhoI
promoter	EF1a
sequencing_primer_3	G-CSF-R (GATGGGGAACACTGCTGTTTA)
sequencing_primer_5	EF-1aF
site_3_destroyed	1
site_5_destroyed

entrez_gene

aliases	IIAE6, MyD88-3, PRVTIRB, TICAM-1, TRIF
gene	TICAM1
id	148022

genbank_ids

NM_182919.3

NP_891549.1

mutation

name

Trif

shRNA_sequence

size

2200

species

9606

Homo sapiens