This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
TAL3151
catalog :
41325
citations: 1
Reference
Sander J, Cade L, Khayter C, Reyon D, Peterson R, Joung J, et al. Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nat Biotechnol. 2011;29:697-8 pubmed publisher
product information
Catalog Number :
41325
Product Name :
TAL3151
article :
doi10.1038/nbt.1934
id4619
pubmed_id21822241
bacterial resistance :
Ampicillin
cloning :
backboneJDS74
backbone_mutation
backbone_originAddgene plasmid #32288
backbone_size6447
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Other
T7
growth notes :
Target binding site: TGCTCTGGTTATGCGGGG It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb.
origin :
37
pi :
alt_names
rasa1b
cloning
clone_methodRestriction Enzyme
cloning_site_3N/A
cloning_site_5N/A
promoterCMV
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasessi:dkey-169a9.2
generasa1b
id564822
genbank_ids
mutation
nameZebrafishCommunity-rasa1b-Right
shRNA_sequence
size1640
species
7955
Danio rerio
tags
locationN terminal on backbone
tag3X Flag
locationC terminal on backbone
tagWT FOKI
resistance markers :
236
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA