pcDNA3 PBD HK-AA (Nigg AH13) | Addgene 41161 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA3 PBD HK-AA (Nigg AH13)

catalog :

41161

citations: 2

Reference
Inoko A, Yano T, Miyamoto T, Matsuura S, Kiyono T, Goshima N, et al. Albatross/FBF1 contributes to both centriole duplication and centrosome separation. Genes Cells. 2018;23:1023-1042 pubmed publisher
Hanisch A, Wehner A, Nigg E, Sillje H. Different Plk1 functions show distinct dependencies on Polo-Box domain-mediated targeting. Mol Biol Cell. 2006;17:448-59 pubmed

product information

Catalog Number :

41161

Product Name :

pcDNA3 PBD HK-AA (Nigg AH13)

article :

doi	10.1091/mbc.e05-08-0801
id	6028
pubmed_id	16267267

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3.1/3x myc-C

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5500

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Addgene plasmid #41162: Plk1-PBDWT (aa 326 603) was cloned in-frame by PCR into a pcDNA3.1 vector encoding an amino-terminal 3xmyc-tag (Invitrogen, Carlsbad, CA) using primers 5'-CCG GAA TTC CAG ATC TTC GAT TGC TCC CAG CAG CCT GG-3' and 5'-CCG CTC GAG TTA GGA GGC CTT GAG ACG GTT GC-3'. The Plk1-PBDAA mutant (H538A, K540A) was made by site-directed mutagenesis using the following primers: 5'-CAA TTC TCC GGA GCC CCG CCT ATC TGT CCC-3' and 5'-GGG ACA GAT AGG CGG GGC TCC GGA GAA TTG-3'.

origin :

pi :

alt_names

Plk1-PBD HK-AA

Plk1 Polo Box Domain

Polo-like kinase 1 Polo Box Domain

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	EcoRI
promoter	CMV
sequencing_primer_3	BGH-rev
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	PLK, STPK13
gene	PLK1
id	5347

genbank_ids

NM_005030.3

X73458.1

mutation

Contains Plk1-PBD only (aa 326 603); HK-AA (H538A, K540A)

name

PBD HK-AA

shRNA_sequence

size

831

species

9606

Homo sapiens