This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3 Lats1 (Nigg HS189)
catalog :
41156
citations: 5
Reference
Ali M, Khramushin A, Yadav V, Schueler Furman O, Ivarsson Y. Elucidation of Short Linear Motif-Based Interactions of the FERM Domains of Ezrin, Radixin, Moesin, and Merlin. Biochemistry. 2023;62:1594-1607 pubmed publisher
Slemmons K, Yeung C, Baumgart J, Juarez J, McCalla A, Helman L. Targeting Hippo-Dependent and Hippo-Independent YAP1 Signaling for the Treatment of Childhood Rhabdomyosarcoma. Cancer Res. 2020;80:3046-3056 pubmed publisher
Yagi H, Onoyama I, Asanoma K, Hori E, Yasunaga M, Kodama K, et al. Gα13-mediated LATS1 down-regulation contributes to epithelial-mesenchymal transition in ovarian cancer. FASEB J. 2019;33:13683-13694 pubmed publisher
Qu L, Wu Z, Li Y, Xu Z, Liu B, Liu F, et al. A feed-forward loop between lncARSR and YAP activity promotes expansion of renal tumour-initiating cells. Nat Commun. 2016;7:12692 pubmed publisher
Chan E, Nousiainen M, Chalamalasetty R, Schäfer A, Nigg E, Sillje H. The Ste20-like kinase Mst2 activates the human large tumor suppressor kinase Lats1. Oncogene. 2005;24:2076-86 pubmed
product information
Catalog Number :
41156
Product Name :
pcDNA3 Lats1 (Nigg HS189)
article :
doi10.1038/sj.onc.1208445
id6025
pubmed_id15688006
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1/3x myc-C
backbone_mutation
backbone_originInvitrogen
backbone_size5500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Human LATS1 cDNA, a kind gift from Hideyuki Saya, was cloned into pcDNA3.1/3x myc-C encoding an amino-terminal 3 x myc tag and verified by sequencing.
origin :
37
pi :
alt_names
LATS, large tumor suppressor, homolog 1
WARTS
wts
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5BamHI/BglII
promoterCMV
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesWARTS, wts
geneLATS1
id9113
genbank_ids
NM_001270519.1
NM_004690.3;
mutation
nameLats1
shRNA_sequence
size3300
species
9606
Homo sapiens
tags
locationN terminal on backbone
tag3xMyc
locationN terminal on backbone
tagPreScission Site
plasmid copy :
Human LATS1 cDNA from Hideyuki Saya.
resistance markers :
1396
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA