This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pFLAG-4S Tetherin
catalog :
41073
citations: 1
Reference
Hammonds J, Ding L, Chu H, Geller K, Robbins A, Wang J, et al. The tetherin/BST-2 coiled-coil ectodomain mediates plasma membrane microdomain localization and restriction of particle release. J Virol. 2012;86:2259-72 pubmed publisher
product information
Catalog Number :
41073
Product Name :
pFLAG-4S Tetherin
article :
doi10.1128/JVI.05906-11
id6002
pubmed_id22130541
bacterial resistance :
Ampicillin
cloning :
backbonepFLAG-Hsp90
backbone_mutationFrom pcDNA3: in-frame N-terminal FLAG epitope added, Hsp90 cds added. From pFLAG-Hsp90 : Hsp90 cds replaced with Tetherin cds by BamHI and XhoI digestion.
backbone_originLen Neckers Laboratory (NCI, Bethesda, MD) (Koga et al. 2006, PMID: 16844778)
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
This plasmid was generated by cloning of tetherin from plasmid pCMV-HA.Tetherin (Addgene plasmid #41068) into the BamHI and XhoI sites of pFLAG-Hsp90 to generate pFLAG-Tetherin (Addgene plasmid #41070). Serine substitutions at positions aa74, aa84, aa137, and aa144 were then generated by Quik Change II Site-directed Mutagenesis Kit (Strategene).
origin :
37
pi :
alt_names
BST-2
bone marrow stromal cell antigen 2
CD317
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5BamHI
promoterCMV
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCD317, HM1.24, TETHERIN
geneBST2
id684
genbank_ids
NM_004335.2
NP_004326.1
mutationS74, S84, 137S, and 144S
nameTetherin
shRNA_sequence
size550
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagFLAG
plasmid copy :
Plasmid pFLAG-Hsp90 from the laboratory of Len Neckers (NCI, Bethesda, MD) (Koga et al. 2006, PMID: 16844778)
resistance markers :
1389
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA